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Tissue-engineered anterior segment eye cultures demonstrate hallmarks of conventional organ culture

Waxman, Susannah ; Strzalkowska, Alicja ; Wang, Chao ; Loewen, Ralitsa ; Dang, Yalong ; Loewen, Nils A.

Graefe's archive for clinical and experimental ophthalmology, 2023-05, Vol.261 (5), p.1359-1368 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

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Título:
Tissue-engineered anterior segment eye cultures demonstrate hallmarks of conventional organ culture
Autor: Waxman, Susannah ; Strzalkowska, Alicja ; Wang, Chao ; Loewen, Ralitsa ; Dang, Yalong ; Loewen, Nils A.
Assuntos: Animals ; Anterior Eye Segment - pathology ; Antibiotics ; Aqueous Humor - physiology ; Archives & records ; Biocompatibility ; Cell culture ; Cells ; Extracellular matrix ; Eye ; Feasibility studies ; Freeze-thawing ; Glaucoma ; Glaucoma - pathology ; Intraocular Pressure ; Medicine ; Medicine & Public Health ; Ophthalmology ; Organ culture ; Organ Culture Techniques ; Perfusion ; Phenotypes ; Physiology ; Pressure ; Structure-function relationships ; Surfactants ; Swine ; Tissue engineering ; Trabecular Meshwork - pathology
É parte de: Graefe's archive for clinical and experimental ophthalmology, 2023-05, Vol.261 (5), p.1359-1368
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Background Glaucoma is a blinding disease largely caused by dysregulation of outflow through the trabecular meshwork (TM), resulting in elevated intraocular pressure (IOP). We hypothesized that transplanting TM cells into a decellularized, tissue-engineered anterior segment eye culture could restore the outflow structure and function. Methods Porcine eyes were decellularized with freeze–thaw cycles and perfusion of surfactant. We seeded control scaffolds with CrFK cells transduced with lentiviral vectors to stably express eGFP and compared them to scaffolds seeded with primary TM cells as well as to normal, unaltered eyes. We tracked the repopulation behavior, performed IOP maintenance challenges, and analyzed the histology. Results Transplanted cells localized to the TM and progressively infiltrated the extracellular matrix, reaching a distribution comparable to normal, unaltered eyes. After a perfusion rate challenge to mimic a glaucomatous pressure elevation, transplanted and normal eyes reestablished a normal intraocular pressure (transplanted = 16.5 ± 0.9 mmHg, normal = 16.9 ± 0.9). However, eyes reseeded with eGFP-expressing CrFK cells could not regulate IOP, remaining high and unstable (27.0 ± 6.2 mmHg) instead. Conclusion Tissue-engineered anterior segment scaffolds can serve as readily available, scalable ocular perfusion cultures. This could reduce dependency on scarce donor globes in outflow research and may allow engineering perfusion cultures with specific geno- and phenotypes.
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

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Tissue-engineered anterior segment eye cultures demonstrate hallmarks of conventional organ culture

Waxman, Susannah ; Strzalkowska, Alicja ; Wang, Chao ; Loewen, Ralitsa ; Dang, Yalong ; Loewen, Nils A.

Berlin/Heidelberg: Springer Berlin Heidelberg

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