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Calcium imaging of muscle cells treated with snake myotoxins reveals toxin synergism and presence of acceptors

Cintra-Francischinelli, M ; Pizzo, P ; Rodrigues-Simioni, L ; Ponce-Soto, L. A ; Rossetto, O ; Lomonte, B ; Gutiérrez, J. M ; Pozzan, T ; Montecucco, C

Cellular and molecular life sciences : CMLS, 2009-05, Vol.66 (10), p.1718-1728 [Periódico revisado por pares]

Basel: Basel : Birkhäuser-Verlag

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Título:
Calcium imaging of muscle cells treated with snake myotoxins reveals toxin synergism and presence of acceptors
Autor: Cintra-Francischinelli, M ; Pizzo, P ; Rodrigues-Simioni, L ; Ponce-Soto, L. A ; Rossetto, O ; Lomonte, B ; Gutiérrez, J. M ; Pozzan, T ; Montecucco, C
Assuntos: Animals ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Bothrops ; Calcium ; Calcium (intracellular) ; Calcium - metabolism ; Calcium imaging ; Calcium influx ; Calcium permeability ; Cell Biology ; Cell death ; Cell Line ; Cell Survival - drug effects ; Cells ; Crotalid Venoms - analysis ; Crotalid Venoms - isolation & purification ; Crotalid Venoms - pharmacology ; Crotalid Venoms - toxicity ; Cytotoxicity ; Life Sciences ; Membrane permeability ; Murinae ; Muscle Fibers, Skeletal - cytology ; Muscle Fibers, Skeletal - drug effects ; Muscle Fibers, Skeletal - metabolism ; Muscles ; Muscular system ; Myoblasts ; Myoblasts - cytology ; Myoblasts - drug effects ; Myoblasts - metabolism ; Myotoxins ; Myotubes ; Phospholipase A2 ; Phospholipids ; PLA2 ; Plasma membranes ; Research Article ; Snake myotoxins ; Snakes ; Studies ; Toxins ; Venom
É parte de: Cellular and molecular life sciences : CMLS, 2009-05, Vol.66 (10), p.1718-1728
Notas: http://dx.doi.org/10.1007/s00018-009-9053-2
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Descrição: Snake myotoxins have a great impact on human health worldwide. Most of them adopt a phospholipase A2 fold and occur in two forms which often co-exist in the same venom: the Asp49 toxins hydrolyse phospholipids, whilst Lys49 toxins are enzymatically inactive. To gain insights into their mechanism of action, muscle cells were exposed to Bothrops myotoxins, and cytosolic Ca²⁺ and cytotoxicity were measured. In both myoblasts and myotubes, the myotoxins induced a rapid and transient rise in cytosolic [Ca²⁺], derived from intracellular stores, followed, only in myotubes, by a large Ca²⁺ influx and extensive cell death. Myoblast viability was unaffected. Notably, in myotubes Asp49 and Lys49 myotoxins acted synergistically to increase the plasma membrane Ca²⁺ permeability, inducing cell death. Therefore, these myotoxins may bind to acceptor(s) coupled to intracellular Ca²⁺ mobilization in both myoblasts and myotubes. However, in myotubes only, the toxins alter plasma membrane permeability, leading to death.
Editor: Basel: Basel : Birkhäuser-Verlag
Idioma: Inglês

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Calcium imaging of muscle cells treated with snake myotoxins reveals toxin synergism and presence of acceptors

Cintra-Francischinelli, M ; Pizzo, P ; Rodrigues-Simioni, L ; Ponce-Soto, L. A ; Rossetto, O ; Lomonte, B ; Gutiérrez, J. M ; Pozzan, T ; Montecucco, C

Basel: Basel : Birkhäuser-Verlag

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