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Interaction of Mre11 and Rad50: two proteins required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae

Johzuka, K. (Osaka University, Toyonaka, Osaka (Japan)) ; Ogawa, H

Genetics (Austin), 1995-04, Vol.139 (4), p.1521-1532 [Periódico revisado por pares]

United States: Genetics Soc America

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Título:
Interaction of Mre11 and Rad50: two proteins required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae
Autor: Johzuka, K. (Osaka University, Toyonaka, Osaka (Japan)) ; Ogawa, H
Assuntos: ADN ; Amino Acid Sequence ; Amino acids ; ARN MENSAJERO ; ARN MESSAGER ; Base Sequence ; Cloning, Molecular ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; DNA Repair - genetics ; DNA, Recombinant ; DNA-Binding Proteins ; Endodeoxyribonucleases ; Exodeoxyribonucleases ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; GENE ; GENES ; Genetics ; Investigations ; MEIOSE ; MEIOSIS ; Meiosis - genetics ; Molecular Sequence Data ; MUTANT ; MUTANTES ; Mutation ; PROTEINAS ; PROTEINE ; Proteins ; Recombination, Genetic ; RNA, Messenger - genetics ; SACCHAROMYCES CEREVISIAE ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins ; SECUENCIA NUCLEICA ; SEQUENCE NUCLEIQUE ; Transcription, Genetic ; Yeast
É parte de: Genetics (Austin), 1995-04, Vol.139 (4), p.1521-1532
Notas: 9553839
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Descrição: A temperature-sensitive mre11-1 mutation of Saccharomyces cerevisiae causes defects in meiotic recombination and DNA repair during vegetative growth at a restrictive temperature. We cloned the MRE11 gene and found that it encodes a 643-amino acid protein with a highly acidic region containing a heptad repeat of Asp at its C-terminus and is located downstream of YMR44 near the RNA1 locus on the right arm of chromosome XIII. Transcripts of the MRE11 gene increased transiently and showed the same kinetics as that of the RAD50 gene during meiosis. In a mre11 disruption mutant (mre11::hisG), meiosis-specific double-strand break (DSB) formation is abolished. A comparison of the properties of mre11:: hisG and a rad50 deletion mutant (rad50 delta) indicated that both mutants exhibited similar phenotypes in both meiosis and mitosis. Characterization of two double mutants, mre11:: hisG rad50 and mre11:: hisG rad50 delta, showed that MRE11 and RAD50 belong to the same epistasis group with respect to meiotic DSB formation and mitotic DNA repair. Using a two-hybrid system, we found that Mre11 interacts with Rad50 and itself in vivo. These results suggest that Mre11 and Rad50 proteins work in a complex in DSB formation and DNA repair during vegetative growth
Editor: United States: Genetics Soc America
Idioma: Inglês

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Interaction of Mre11 and Rad50: two proteins required for DNA repair and meiosis-specific double-strand break formation in Saccharomyces cerevisiae

Johzuka, K. (Osaka University, Toyonaka, Osaka (Japan)) ; Ogawa, H

United States: Genetics Soc America

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