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MN/CA IX/G250 as a potential target for immunotherapy of renal cell carcinomas

UEMURA, H ; NAKAGAWA, Y ; YOSHIDA, K ; SAGA, S ; YOSHIKAWA, K ; HIRAO, Y ; OOSTERWIJK, E

British Journal of Cancer, 1999-10, Vol.81 (4), p.741-746 [Periódico revisado por pares]

Basingstoke: Nature Publishing Group

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Título:
MN/CA IX/G250 as a potential target for immunotherapy of renal cell carcinomas
Autor: UEMURA, H ; NAKAGAWA, Y ; YOSHIDA, K ; SAGA, S ; YOSHIKAWA, K ; HIRAO, Y ; OOSTERWIJK, E
Assuntos: Antigens, Neoplasm - analysis ; Antineoplastic agents ; Biological and medical sciences ; Biomarkers, Tumor - analysis ; Carbonic Anhydrase IX ; Carbonic Anhydrases ; Carcinoma, Renal Cell - chemistry ; Carcinoma, Renal Cell - diagnosis ; Humans ; Immunotherapy ; Kidney Neoplasms - chemistry ; Kidney Neoplasms - diagnosis ; Medical sciences ; Neoplasm Proteins - analysis ; Neoplasm Proteins - genetics ; Pharmacology. Drug treatments ; Regular ; Reverse Transcriptase Polymerase Chain Reaction ; Tumor Cells, Cultured
É parte de: British Journal of Cancer, 1999-10, Vol.81 (4), p.741-746
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: The monoclonal antibody G250 (mAbG250) raised against a human renal cell carcinoma (RCC) has been shown to react with a large number of RCCs. Recently, G250 antigen was isolated and found to be homologous to the MN/CA9 gene originally identified in HeLa cells. To determine whether G250 antigen (MN/CA IX/G250) could be a potential therapeutic target and a tumour marker, a total of 147 cases of RCC were investigated immunohistochemically as well as by reverse transcriptase polymerase chain reaction (RT-PCR) analysis. In addition, total RNAs extracted from patients' peripheral blood samples were analysed for MN/CA9/G250 mRNA signals. Immunohistochemistry demonstrated strong expression in 128/147 (87.1%) of RCCs, in contrast to the lack of expression observed in normal tissues. RT-PCR analyses of frozen specimens resulted in the clear detection of MN/CA9/G250 mRNA signals in 137/147 (93.2%), and despite subtle differences the results were almost identical to those for immunohistochemistry. Although high-grade and -stage tumours exhibited significantly lower expression than low-grade and -stage tumours, a large proportion of tumours expressed MN/G250 protein as well as mRNA. RT-PCR analysis of patients' blood samples revealed the presence of circulating MN/CA9/G250 expressing cells. These findings suggest that this antigen may be a potential therapeutic target as well as diagnostic marker for RCCs.
Editor: Basingstoke: Nature Publishing Group
Idioma: Inglês

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MN/CA IX/G250 as a potential target for immunotherapy of renal cell carcinomas

UEMURA, H ; NAKAGAWA, Y ; YOSHIDA, K ; SAGA, S ; YOSHIKAWA, K ; HIRAO, Y ; OOSTERWIJK, E

Basingstoke: Nature Publishing Group

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