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Nuclear Activity of ROXY1, a Glutaredoxin Interacting with TGA Factors, Is Required for Petal Development in Arabidopsis thaliana

Li, Shutian ; Lauri, Andrea ; Ziemann, Mark ; Busch, Andrea ; Bhave, Mrinal ; Zachgo, Sabine

The Plant cell, 2009-02, Vol.21 (2), p.429-441 [Periódico revisado por pares]

England: American Society of Plant Biologists

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Título:
Nuclear Activity of ROXY1, a Glutaredoxin Interacting with TGA Factors, Is Required for Petal Development in Arabidopsis thaliana
Autor: Li, Shutian ; Lauri, Andrea ; Ziemann, Mark ; Busch, Andrea ; Bhave, Mrinal ; Zachgo, Sabine
Assuntos: Amino Acid Sequence ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis - metabolism ; Arabidopsis Proteins - analysis ; Arabidopsis Proteins - chemistry ; Arabidopsis Proteins - physiology ; Arabidopsis thaliana ; Cell Nucleus - metabolism ; Chemical properties ; Flowers ; Flowers - genetics ; Flowers - growth & development ; Flowers - metabolism ; Gene Deletion ; Gene expression regulation ; Genes ; Genetic aspects ; Glutaredoxins - analysis ; Glutaredoxins - chemistry ; Glutaredoxins - physiology ; Growth ; Luminescent Proteins - analysis ; Molecular Sequence Data ; Morphogenesis ; Mutagenesis, Site-Directed ; Nicotiana - genetics ; Petals ; Phenotype ; Phenotypes ; Phylogeny ; Physiological aspects ; Plant interaction ; Plants ; Proteins ; Recombinant Proteins - analysis ; Sequence Alignment ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Two-Hybrid System Techniques ; Yeasts
É parte de: The Plant cell, 2009-02, Vol.21 (2), p.429-441
Notas: These authors contributed equally to this work.
Online version contains Web-only data.
Current address: Parco Tecnologico Padano via Einstein, 26900 Lodi, Italy.
www.plantcell.org/cgi/doi/10.1105/tpc.108.064477
Address correspondence to zachgo@biologie.uni-osnbrueck.de.
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Sabine Zachgo (zachgo@biologie.uni-osnbrueck.de).
Descrição: Glutaredoxins (GRXs) have thus far been associated mainly with redox-regulated processes participating in stress responses. However, ROXY1, encoding a GRX, has recently been shown to regulate petal primorida initiation and further petal morphogenesis in Arabidopsis thaliana. ROXY1 belongs to a land plant-specific class of GRXs that has a CC-type active site motif, which deviates from ubiquitously occurring CPYC and CGFS GRXs. Expression studies of yellow fluorescent protein-ROXY1 fusion genes driven by the cauliflower mosaic virus 35S promoter reveal a nucleocytoplasmic distribution of ROXY1. We demonstrate that nuclear localization of ROXY1 is indispensable and thus crucial for its activity in flower development. Yeast two-hybrid screens identified TGA transcription factors as interacting proteins, which was confirmed by bimolecular fluorescence complementation experiments showing their nuclear interaction in planta. Overlapping expression patterns of ROXY1 and TGA genes during flower development demonstrate that ROXY1/TGA protein interactions can occur in vivo and support their biological relevance in petal development. Deletion analysis of ROXY1 demonstrates the importance of the C terminus for its functionality and for mediating ROXY1/TGA protein interactions. Phenotypic analysis of the roxy1-2 pan double mutant and an engineered chimeric repressor mutant from PERIANTHIA (PAN), a floral TGA gene, supports a dual role of ROXY1 in petal development. Together, our results show that the ROXY1 protein functions in the nucleus, likely by modifying PAN posttranslationally and thereby regulating its activity in petal primordia initiation. Additionally, ROXY1 affects later petal morphogenesis, probably by modulating other TGA factors that might act redundantly during differentiation of second whorl organs.
Editor: England: American Society of Plant Biologists
Idioma: Inglês

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Nuclear Activity of ROXY1, a Glutaredoxin Interacting with TGA Factors, Is Required for Petal Development in Arabidopsis thaliana

Li, Shutian ; Lauri, Andrea ; Ziemann, Mark ; Busch, Andrea ; Bhave, Mrinal ; Zachgo, Sabine

England: American Society of Plant Biologists

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