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Dicer is required for female reproductive tract development and fertility in the mouse

Gonzalez, Gabriel ; Behringer, Richard R.

Molecular reproduction and development, 2009-07, Vol.76 (7), p.678-688 [Revista revisada por pares]

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

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Dicer is required for female reproductive tract development and fertility in the mouse
Autor: Gonzalez, Gabriel ; Behringer, Richard R.
Materias: Animals ; Biological and medical sciences ; DEAD-box RNA Helicases - genetics ; DEAD-box RNA Helicases - physiology ; Endoribonucleases - genetics ; Endoribonucleases - physiology ; Estrogens - blood ; Female ; Fertility ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genitalia, Female - embryology ; Genitalia, Female - growth & development ; Genitalia, Female - metabolism ; Genitalia, Female - pathology ; Hormone metabolism and regulation ; Immunohistochemistry ; Infertility, Female - genetics ; Infertility, Female - metabolism ; Inflammation ; Integrases - genetics ; Integrases - metabolism ; Mammalian female genital system ; Mice ; Mice, Transgenic ; Mullerian Ducts - embryology ; Mullerian Ducts - metabolism ; Oviducts - growth & development ; Oviducts - pathology ; Progesterone - blood ; Ribonuclease III ; Vertebrates: reproduction ; Wnt Proteins - genetics ; Wnt Proteins - metabolism
Es parte de: Molecular reproduction and development, 2009-07, Vol.76 (7), p.678-688
Notas: istex:CE26ED0EBADFBDB21369E47AA177664CE9177067
National Institutes of Health - No. HD30284
Cancer Center Support - No. CA16672
ark:/67375/WNG-1P3RZN0K-3
ArticleID:MRD21010
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descripción: Dicer encodes a riboendonuclease required for microRNA biosynthesis. Dicer was inactivated in Müllerian duct mesenchyme‐derived tissues of the reproductive tract of the mouse, using an Amhr2‐Cre allele. Although Amhr2‐Cre; Dicer conditional mutant males appeared normal and were fertile, mutant females were infertile. In adult mutant females, there was a reduction in the size of the oviducts and uterine horns. The oviducts were less coiled compared to controls and cysts formed at the isthmus near the uterotubal junction. Unfertilized, degenerate oocytes were commonly found within these cysts, indicating a defect in embryo transit. Beads transferred into the mutant oviduct failed to migrate into the uterus. In addition, blastocysts transferred directly into the mutant uterus did not result in pregnancy. Histological analysis demonstrated that the mutant uterus contained less glandular tissue and often the few glands that remained were found within the myometrium, an abnormal condition known as adenomyosis. In adult mutants, there was ectopic expression of Wnt4 and Wnt5a in the luminal epithelium (LE) and glandular epithelium (GE) of the uterus, and Wnt11 was ectopically expressed in GE. These results demonstrate that Dicer is necessary for postnatal differentiation of Müllerian duct mesenchyme‐derived tissues of the female reproductive tract, suggesting that microRNAs are important regulators of female reproductive tract development and fertility. Mol. Reprod. Dev. 76: 678–688, 2009. © 2009 Wiley‐Liss, Inc.
Editor: Hoboken: Wiley Subscription Services, Inc., A Wiley Company
Idioma: Inglés

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Dicer is required for female reproductive tract development and fertility in the mouse

Gonzalez, Gabriel ; Behringer, Richard R.

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

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