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Identification of Conus Peptidylprolyl Cis-Trans Isomerases (PPIases) and Assessment of Their Role in the Oxidative Folding of Conotoxins

Safavi-Hemami, Helena ; Bulaj, Grzegorz ; Olivera, Baldomero M. ; Williamson, Nicholas A. ; Purcell, Anthony W.

The Journal of biological chemistry, 2010-04, Vol.285 (17), p.12735-12746 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
Identification of Conus Peptidylprolyl Cis-Trans Isomerases (PPIases) and Assessment of Their Role in the Oxidative Folding of Conotoxins
Autor: Safavi-Hemami, Helena ; Bulaj, Grzegorz ; Olivera, Baldomero M. ; Williamson, Nicholas A. ; Purcell, Anthony W.
Assuntos: Animals ; Base Sequence ; Conotoxins - biosynthesis ; Conotoxins - genetics ; Conus ; Conus Snail - genetics ; Conus Snail - metabolism ; Drosophila ; Endoplasmic Reticulum - genetics ; Endoplasmic Reticulum - metabolism ; Enzymes ; Genomics and Proteomics ; Human immunodeficiency virus 1 ; Humans ; Isoenzymes - genetics ; Isoenzymes - metabolism ; Marine ; Molecular Sequence Data ; Multigene Family - physiology ; Oxidation-Reduction ; Peptides ; Peptides - genetics ; Peptides - metabolism ; Peptides/Biosynthesis ; Peptides/Conformation ; Peptidylprolyl Isomerase - genetics ; Peptidylprolyl Isomerase - metabolism ; Proline - genetics ; Proline - metabolism ; Protein Folding ; Protein Structure and Folding ; Protein/Prolyl Isomerase ; Proteomics ; Toxins/Drugs/Xenobiotics/Venom
É parte de: The Journal of biological chemistry, 2010-04, Vol.285 (17), p.12735-12746
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Peptidylprolyl cis-trans isomerases (PPIases) are ubiquitous proteins that catalyze the cis-trans isomerization of prolines. A number of proteins, such as Drosophila rhodopsin and the human immunodeficiency viral protein HIV-1 Gag, have been identified as endogenous substrates for PPIases. However, very little is known about the interaction of PPIases with small, disulfide-rich peptides. Marine cone snails synthesize a wide array of cysteine-rich peptides, called conotoxins, many of which contain one or more prolines or hydroxyprolines. To identify whether PPIase-associated cis-trans isomerization of these residues affects the oxidative folding of conotoxins, we identified, sequenced, and expressed three functionally active isoforms of PPIase from the venom gland of Conus novaehollandiae, and we characterized their ability to facilitate oxidative folding of conotoxins in vitro. Three conotoxins, namely μ-GIIIA, μ-SIIIA, and ω-MVIIC, derived from two distinct toxin gene families were assayed. Conus PPIase significantly increased the rate of appearance of the native form of μ-GIIIA, a peptide containing three hydroxyprolines. In contrast, the presence of PPIase had no effect on the folding of μ-SIIIA and ω-MVIIC, peptides containing no or one proline residue, respectively. We further showed that an endoplasmic reticulum-resident PPIase isoform facilitated folding of μ-GIIIA more efficiently than two cytosolic isoforms. This is the first study to demonstrate PPIase-assisted folding of conotoxins, small disulfide-rich peptides with unique structural properties.
Editor: United States: Elsevier Inc
Idioma: Inglês

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Identification of Conus Peptidylprolyl Cis-Trans Isomerases (PPIases) and Assessment of Their Role in the Oxidative Folding of Conotoxins

Safavi-Hemami, Helena ; Bulaj, Grzegorz ; Olivera, Baldomero M. ; Williamson, Nicholas A. ; Purcell, Anthony W.

United States: Elsevier Inc

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