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Fixation methods for electron microscopy of human and other liver

Wisse, Eddie ; Braet, Filip ; Duimel, Hans ; Vreuls, Celien ; Koek, Ger ; Olde Damink, Steven W M ; van den Broek, Maartje A J ; De Geest, Bart ; Dejong, Cees H C ; Tateno, Chise ; Frederik, Peter

World journal of gastroenterology : WJG, 2010-06, Vol.16 (23), p.2851-2866

United States: Department of Internal Medicine, University of Maastricht, 6200 MD Maastricht, The Netherlands%Australian Key Center for Microscopy and Microanalysis, Sydney University, NSW 2006 Sydney,Australia%Electron Microscope Unit, University of Maastricht, 6200 MD Maastricht, The Netherlands%Department of Internal Medicine, University of Maastricht, 6200 MD Maastricht, The Netherlands%Department of Surgery, University of Maastricht, 6200 MD Maastricht, The Netherlands%Center for Molecular and Vascular Biology,University of Leuven, 3000 Leuven, Belgium%PhoenixBio Co., 3-4-1 Kagamiyama, Higashi-Hiroshima 739-0046, Japan

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  • Título:
    Fixation methods for electron microscopy of human and other liver
  • Autor: Wisse, Eddie ; Braet, Filip ; Duimel, Hans ; Vreuls, Celien ; Koek, Ger ; Olde Damink, Steven W M ; van den Broek, Maartje A J ; De Geest, Bart ; Dejong, Cees H C ; Tateno, Chise ; Frederik, Peter
  • Assuntos: Animals ; Biopsy ; Cells, Cultured ; Guidelines For Basic Science ; Histocytological Preparation Techniques - methods ; Humans ; Liver - ultrastructure ; Microscopy, Electron ; Microscopy, Electron, Scanning ; Microscopy, Electron, Transmission ; Perfusion ; Tissue Fixation - methods
  • É parte de: World journal of gastroenterology : WJG, 2010-06, Vol.16 (23), p.2851-2866
  • Notas: Perfusion
    Biopsy
    14-1219/R
    Liver
    R575
    Liver; Tissue fixation; Perfusion; Electron microscopy; Biopsy
    Electron microscopy
    Tissue fixation
    ObjectType-Article-2
    SourceType-Scholarly Journals-1
    ObjectType-Feature-3
    content type line 23
    ObjectType-Review-1
    Correspondence to: Dr. Eddie Wisse, Professor, Electron Microscope Unit, University of Maastricht, PO Box 616, 6200 MD Maastricht, The Netherlands. eddie@wisse.be
    Author contributions: Wisse E and Braet F designed the fixation procedures and wrote the manuscript together; Wisse E performed the EM observations; Braet F provided the schematic diagrams; Duimel H and Vreuls C prepared the specimens and sections and provided technical support; Olde Damink SWM, Dejong CHC, van den Broek MAJ and Koek G provided the human liver wedge biopsies, obtained approval of the ethical committee and provided the clinical data; De Geest B provided rabbit, mouse and human livers and biopsies; Tateno C provided mouse livers of different strains; Frederik P supervised the study as head of the EM Unit.
    Telephone: +31-43-3881280 Fax: +31-43-3671040
  • Descrição: For an electron microscopic study of the liver,expertise and complicated,time-consuming processing of hepatic tissues and cells is needed.The interpretation of electron microscopy(EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation,embedding,sectioning,contrast staining and microscopic imaging.Hence,the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue,for the purpose of preserv...
  • Editor: United States: Department of Internal Medicine, University of Maastricht, 6200 MD Maastricht, The Netherlands%Australian Key Center for Microscopy and Microanalysis, Sydney University, NSW 2006 Sydney,Australia%Electron Microscope Unit, University of Maastricht, 6200 MD Maastricht, The Netherlands%Department of Internal Medicine, University of Maastricht, 6200 MD Maastricht, The Netherlands%Department of Surgery, University of Maastricht, 6200 MD Maastricht, The Netherlands%Center for Molecular and Vascular Biology,University of Leuven, 3000 Leuven, Belgium%PhoenixBio Co., 3-4-1 Kagamiyama, Higashi-Hiroshima 739-0046, Japan
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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