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Neurokinin B Stimulates GnRH Release in the Male Monkey (Macaca mulatta) and Is Colocalized with Kisspeptin in the Arcuate Nucleus

Ramaswamy, Suresh ; Seminara, Stephanie B ; Ali, Barkat ; Ciofi, Philippe ; Amin, Nisar A ; Plant, Tony M

Endocrinology (Philadelphia), 2010-09, Vol.151 (9), p.4494-4503 [Periódico revisado por pares]

Chevy Chase, MD: Endocrine Society

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Título:
Neurokinin B Stimulates GnRH Release in the Male Monkey (Macaca mulatta) and Is Colocalized with Kisspeptin in the Arcuate Nucleus
Autor: Ramaswamy, Suresh ; Seminara, Stephanie B ; Ali, Barkat ; Ciofi, Philippe ; Amin, Nisar A ; Plant, Tony M
Assuntos: Animals ; Arcuate Nucleus of Hypothalamus - drug effects ; Arcuate Nucleus of Hypothalamus - metabolism ; Biological and medical sciences ; Drug Interactions ; Fundamental and applied biological sciences. Psychology ; Gonadotropin-Releasing Hormone - secretion ; Humans ; Immunohistochemistry ; Injections, Intravenous ; Kisspeptins ; Luteinizing Hormone - blood ; Luteinizing Hormone - secretion ; Macaca mulatta ; Male ; Microscopy, Confocal ; Neurokinin B - metabolism ; Neurokinin B - pharmacology ; Oligopeptides - metabolism ; Oligopeptides - pharmacology ; Orchiectomy ; Peptide Fragments - pharmacology ; Quinolines - pharmacology ; Receptors, G-Protein-Coupled - agonists ; Receptors, G-Protein-Coupled - antagonists & inhibitors ; Receptors, G-Protein-Coupled - metabolism ; Receptors, LHRH - antagonists & inhibitors ; Receptors, Neurokinin-3 - agonists ; Receptors, Neurokinin-3 - antagonists & inhibitors ; Receptors, Neurokinin-3 - metabolism ; Substance P - analogs & derivatives ; Substance P - pharmacology ; Vertebrates: endocrinology
É parte de: Endocrinology (Philadelphia), 2010-09, Vol.151 (9), p.4494-4503
Notas: Address all correspondence and requests for reprints to: Dr. Tony M. Plant, Department of Obstetrics, Gynecology, and Reproductive Sciences, B331 Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213. E-mail: plant1@pitt.edu.
Descrição: Human genetics indicate that kisspeptin and neurokinin B (NKB) signaling are necessary for generating pulsatile LH release and therefore for initiation of puberty and maintaining gonadal function. In the present study, male monkeys were employed to examine 1) whether activation of the NKB receptor (NK3R) is associated with GnRH release, and 2) hypothalamic localization of these peptides using immunofluorescence histochemistry. Agonadal juveniles, in which pituitary responsiveness to GnRH was heightened by GnRH priming, were employed to indirectly examine GnRH-releasing actions of NK3R and kisspeptin receptor agonists by tracking LH after their iv injection. Castrated adults were used for immunohistochemistry. Single iv injections of NKB or senktide (an NK3R agonist) elicited robust LH discharges that were abolished by GnRH receptor antagonism (acyline) confirming the ligands’ hypothalamic action. Intermittent infusion of senktide (1-min pulse every hour for 4 h), in contrast to that of kisspeptin, failed to sustain pulsatile GnRH release. Repetitive senktide injections did not compromise the GnRH-releasing action of kisspeptin. NKB and kisspeptin were colocalized in perikarya of the arcuate nucleus and in axonal projections to the median eminence, confirming earlier findings in sheep. These results are consistent with the human genetics, and indicate that although brief activation of NK3R stimulates GnRH release, repetitive stimulation of this pathway, in contrast to that of kisspeptin receptor, fails to sustain pulsatile GnRH release. In addition, the data provide a platform for future elucidation of the interactions between NKB and kisspeptin that are required for generating pulsatile GnRH release in primates. Neurokinin B is found in the monkey hypothalamus, and iv injections of this peptide activate the hypothalamic-pituitary axis.
Editor: Chevy Chase, MD: Endocrine Society
Idioma: Inglês

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Neurokinin B Stimulates GnRH Release in the Male Monkey (Macaca mulatta) and Is Colocalized with Kisspeptin in the Arcuate Nucleus

Ramaswamy, Suresh ; Seminara, Stephanie B ; Ali, Barkat ; Ciofi, Philippe ; Amin, Nisar A ; Plant, Tony M

Chevy Chase, MD: Endocrine Society

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