Idioma:

Inside-out Signaling Promotes Dynamic Changes in the Carcinoembryonic Antigen-related Cellular Adhesion Molecule 1 (CEACAM1) Oligomeric State to Control Its Cell Adhesion Properties

Patel, Prerna C. ; Lee, Hannah S.W. ; Ming, Aaron Y.K. ; Rath, Arianna ; Deber, Charles M. ; Yip, Christopher M. ; Rocheleau, Jonathan V. ; Gray-Owen, Scott D.

The Journal of biological chemistry, 2013-10, Vol.288 (41), p.29654-29669 [Periódico revisado por pares]

United States: Elsevier Inc

Texto completo disponível

Citações Citado por

Enviar para

Título:
Inside-out Signaling Promotes Dynamic Changes in the Carcinoembryonic Antigen-related Cellular Adhesion Molecule 1 (CEACAM1) Oligomeric State to Control Its Cell Adhesion Properties
Autor: Patel, Prerna C. ; Lee, Hannah S.W. ; Ming, Aaron Y.K. ; Rath, Arianna ; Deber, Charles M. ; Yip, Christopher M. ; Rocheleau, Jonathan V. ; Gray-Owen, Scott D.
Assuntos: Amino Acid Motifs - genetics ; Amino Acid Sequence ; Antigens, CD - chemistry ; Antigens, CD - genetics ; Antigens, CD - metabolism ; Bacterial Adhesion ; Calcium - metabolism ; Calcium Signaling ; Calmodulin ; Calmodulin - metabolism ; Cancer ; Carcinoembryonic Antigen-related Cell Adhesion Molecule ; CEACAM1 ; Cell Adhesion ; Cell Adhesion Molecules - chemistry ; Cell Adhesion Molecules - genetics ; Cell Adhesion Molecules - metabolism ; Cell Biology ; Cell Membrane - metabolism ; HeLa Cells ; Homodimer ; Homophilic Binding ; Humans ; Immunoblotting ; Membrane Proteins ; Microscopy, Confocal ; Models, Molecular ; Molecular Sequence Data ; Mutation ; Protein Binding ; Protein Multimerization ; Protein Structure, Tertiary ; Protein Tyrosine Phosphatase, Non-Receptor Type 11 - metabolism ; Protein Tyrosine Phosphatase, Non-Receptor Type 6 - metabolism ; Signal Transduction ; src-Family Kinases - metabolism
É parte de: The Journal of biological chemistry, 2013-10, Vol.288 (41), p.29654-29669
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Recipient of stipend support from the Banting and Best Diabetes Centre, University of Toronto.
Descrição: Cell-cell contacts are fundamental to multicellular organisms and are subject to exquisite levels of control. The carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) can engage in both cis-homophilic (parallel) oligomerization and trans-homophilic (anti-parallel) binding. In this study, we establish that the CEACAM1 transmembrane domain has a propensity to form cis-dimers via the transmembrane-embedded 432GXXXG436 motif and that this basal state is overcome when activated calmodulin binds to the CEACAM1 cytoplasmic domain. Although mutation of the 432GXXXG436 motif reduced CEACAM1 oligomerization, it did not affect surface localization of the receptor or influence CEACAM1-dependent cellular invasion by the pathogenic Neisseria. The mutation did, however, have a striking effect on CEACAM1-dependent cellular aggregation, increasing both the kinetics of cell-cell association and the size of cellular aggregates formed. CEACAM1 association with tyrosine kinase c-Src and tyrosine phosphatases SHP-1 and SHP-2 was not affected by the 432GXXXG436 mutation, consistent with their association with the monomeric form of wild type CEACAM1. Collectively, our results establish that a dynamic oligomer-to-monomer shift in surface-expressed CEACAM1 facilitates trans-homophilic binding and downstream effector signaling. Background: Carcinoembryonic antigen-related cellular adhesion molecules (CEACAMs) engage in intercellular binding and influence cellular growth and differentiation. Results: Calcium-dependent signals cause dissolution of the transmembrane domain-driven basal state CEACAM1 oligomers into monomers that mediate intercellular binding. Conclusion: Regulated switching in CEACAM1 oligomerization controls cell-cell adhesion and downstream effector recruitment. Significance: Inside-out signaling effects CEACAM1-dependent cell adhesion by controlling a reversible dimer-to-monomer transition.
Editor: United States: Elsevier Inc
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 9 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Inside-out Signaling Promotes Dynamic Changes in the Carcinoembryonic Antigen-related Cellular Adhesion Molecule 1 (CEACAM1) Oligomeric State to Control Its Cell Adhesion Properties

Patel, Prerna C. ; Lee, Hannah S.W. ; Ming, Aaron Y.K. ; Rath, Arianna ; Deber, Charles M. ; Yip, Christopher M. ; Rocheleau, Jonathan V. ; Gray-Owen, Scott D.

United States: Elsevier Inc

Buscando em bases de dados remotas. Favor aguardar.