Idioma:

Molecular architecture of Streptococcus pneumoniae surface thioredoxin‐fold lipoproteins crucial for extracellular oxidative stress resistance and maintenance of virulence

Saleh, Malek ; Bartual, Sergio G. ; Abdullah, Mohammed R. ; Jensch, Inga ; Asmat, Tauseef M. ; Petruschka, Lothar ; Pribyl, Thomas ; Gellert, Manuela ; Lillig, Christopher H. ; Antelmann, Haike ; Hermoso, Juan A. ; Hammerschmidt, Sven

EMBO molecular medicine, 2013-12, Vol.5 (12), p.1852-1870 [Periódico revisado por pares]

England: EMBO Press

Texto completo disponível

Citações Citado por

Enviar para

Título:
Molecular architecture of Streptococcus pneumoniae surface thioredoxin‐fold lipoproteins crucial for extracellular oxidative stress resistance and maintenance of virulence
Autor: Saleh, Malek ; Bartual, Sergio G. ; Abdullah, Mohammed R. ; Jensch, Inga ; Asmat, Tauseef M. ; Petruschka, Lothar ; Pribyl, Thomas ; Gellert, Manuela ; Lillig, Christopher H. ; Antelmann, Haike ; Hermoso, Juan A. ; Hammerschmidt, Sven
Assuntos: Animals ; Antimicrobial agents ; Antioxidants ; Bacteria ; Bacterial Proteins - chemistry ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Catalytic Domain ; Crystallography, X-Ray ; Disease Models, Animal ; Enzymes ; Experiments ; Female ; Hydrogen peroxide ; Hydrogen Peroxide - pharmacology ; Lipoproteins ; Macrophages ; Macrophages - immunology ; Macrophages - physiology ; meningitis ; Methionine ; Methionine - analogs & derivatives ; Methionine - pharmacology ; Mice ; Molecular Sequence Data ; Nasopharynx ; Oxidation ; Oxidative stress ; Oxidative Stress - drug effects ; Pathogenesis ; Phagocytosis ; pneumococci ; Pneumonia ; Pneumonia - immunology ; Pneumonia - microbiology ; Pneumonia - mortality ; Protein Structure, Tertiary ; Proteins ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - chemistry ; Recombinant Proteins - genetics ; Reductase ; Streptococcus infections ; Streptococcus pneumoniae ; Streptococcus pneumoniae - metabolism ; Streptococcus pneumoniae - pathogenicity ; Structure-function relationships ; Survival Analysis ; Thioredoxin ; Virulence
É parte de: EMBO molecular medicine, 2013-12, Vol.5 (12), p.1852-1870
Notas: These authors contributed equally to this work.
Descrição: The respiratory pathogen Streptococcus pneumoniae has evolved efficient mechanisms to resist oxidative stress conditions and to displace other bacteria in the nasopharynx. Here we characterize at physiological, functional and structural levels two novel surface‐exposed thioredoxin‐family lipoproteins, Etrx1 and Etrx2. The impact of both Etrx proteins and their redox partner methionine sulfoxide reductase SpMsrAB2 on pneumococcal pathogenesis was assessed in mouse virulence studies and phagocytosis assays. The results demonstrate that loss of function of either both Etrx proteins or SpMsrAB2 dramatically attenuated pneumococcal virulence in the acute mouse pneumonia model and that Etrx proteins compensate each other. The deficiency of Etrx proteins or SpMsrAB2 further enhanced bacterial uptake by macrophages, and accelerated pneumococcal killing by H2O2 or free methionine sulfoxides (MetSO). Moreover, the absence of both Etrx redox pathways provokes an accumulation of oxidized SpMsrAB2 in vivo. Taken together our results reveal insights into the role of two extracellular electron pathways required for reduction of SpMsrAB2 and surface‐exposed MetSO. Identification of this system and its target proteins paves the way for the design of novel antimicrobials. Streptococcus pneumoniae can cause fatal diseases. While cytoplasmic oxidative stress‐resistance is known, pneumococci thioredoxin Etrx1, ‐2 and SpMsrAB2 are shown here to be key for virulence and extracellular oxidative stress resistance mechanism.
Editor: England: EMBO Press
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 7 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Molecular architecture of Streptococcus pneumoniae surface thioredoxin‐fold lipoproteins crucial for extracellular oxidative stress resistance and maintenance of virulence

Saleh, Malek ; Bartual, Sergio G. ; Abdullah, Mohammed R. ; Jensch, Inga ; Asmat, Tauseef M. ; Petruschka, Lothar ; Pribyl, Thomas ; Gellert, Manuela ; Lillig, Christopher H. ; Antelmann, Haike ; Hermoso, Juan A. ; Hammerschmidt, Sven

England: EMBO Press

Buscando em bases de dados remotas. Favor aguardar.