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Antibiosis interaction of Staphylococccus aureus on Aspergillus fumigatus assessed in vitro by mixed biofilm formation

Ramírez Granillo, Adrián ; Canales, María Gabriela Medina ; Espíndola, María Esther Sánchez ; Martínez Rivera, María Angeles ; de Lucio, Victor Manuel Bautista ; Tovar, Aída Verónica Rodríguez

BMC microbiology, 2015-02, Vol.15 (1), p.33-33, Article 33 [Periódico revisado por pares]

England: BioMed Central Ltd

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Título:
Antibiosis interaction of Staphylococccus aureus on Aspergillus fumigatus assessed in vitro by mixed biofilm formation
Autor: Ramírez Granillo, Adrián ; Canales, María Gabriela Medina ; Espíndola, María Esther Sánchez ; Martínez Rivera, María Angeles ; de Lucio, Victor Manuel Bautista ; Tovar, Aída Verónica Rodríguez
Assuntos: Analysis ; Antibiosis - physiology ; Aspergillus fumigatus - growth & development ; Aspergillus fumigatus - isolation & purification ; Aspergillus fumigatus - ultrastructure ; Biofilms - growth & development ; Chitin ; Colony Count, Microbial ; Comparative analysis ; Cornea - microbiology ; Extracellular Matrix - ultrastructure ; Fluorescence microscopy ; Fungal Polysaccharides - chemistry ; Genetic aspects ; Health aspects ; Humans ; Hyphae - growth & development ; Hyphae - ultrastructure ; Keratitis - microbiology ; Keratitis - pathology ; Microscopy, Electron, Scanning ; Physiological aspects ; Polysaccharides, Bacterial - chemistry ; Spores, Fungal - growth & development ; Spores, Fungal - ultrastructure ; Staphylococcus aureus ; Staphylococcus aureus - growth & development ; Staphylococcus aureus - isolation & purification ; Staphylococcus aureus - pathogenicity ; Staphylococcus aureus - ultrastructure ; Staphylococcus aureus infections
É parte de: BMC microbiology, 2015-02, Vol.15 (1), p.33-33, Article 33
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Descrição: Microorganisms of different species interact in several ecological niches, even causing infection. During the infectious process, a biofilm of single or multispecies can develop. Aspergillus fumigatus and Staphyloccocus aureus are etiologic agents that can cause infectious keratitis. We analyzed in vitro single A. fumigatus and S. aureus, and mixed A. fumigatus-S. aureus biofilms. Both isolates were from patients with infectious keratitis. Structure of the biofilms was analyzed through microscopic techniques including scanning electron microscopy (SEM), transmission electron microscopy (TEM), confocal, and fluorescence microscopy (CLSM) in mixed biofilm as compared with the single A. fumigatus biofilm. To our knowledge, this is the first time that the structural characteristics of the mixed biofilm A. fumigatus-A. fumigatus were described and shown. S. aureus sharply inhibited the development of biofilm formed by A. fumigatus, regardless of the stage of biofilm formation and bacterial inoculum. Antibiosis effect of bacterium on fungus was as follows: scarce production of A. fumigatus biofilm; disorganized fungal structures; abortive hyphae; and limited hyphal growth; while conidia also were scarce, have modifications in their surface and presented lyses. Antagonist effect did not depend on bacterial concentration, which could probably be due to cell-cell contact interactions and release of bacterial products. In addition, we present images about the co-localization of polysaccharides (glucans, mannans, and chitin), and DNA that form the extracellular matrix (ECM). In contrast, single biofilms showed extremely organized structures: A. fumigatus showed abundant hyphal growth, hyphal anastomosis, and channels, as well as some conidia, and ECM. S. aureus showed microcolonies and cell-to-cell bridges and ECM. Herein we described the antibiosis relationship of S. aureus against A. fumigatus during in vitro biofilm formation, and report the composition of the ECM formed.
Editor: England: BioMed Central Ltd
Idioma: Inglês

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Antibiosis interaction of Staphylococccus aureus on Aspergillus fumigatus assessed in vitro by mixed biofilm formation

Ramírez Granillo, Adrián ; Canales, María Gabriela Medina ; Espíndola, María Esther Sánchez ; Martínez Rivera, María Angeles ; de Lucio, Victor Manuel Bautista ; Tovar, Aída Verónica Rodríguez

England: BioMed Central Ltd

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