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Stem cells from human amniotic fluid exert immunoregulatory function via secreted indoleamine 2,3‐dioxygenase1

Romani, Rita ; Pirisinu, Irene ; Calvitti, Mario ; Pallotta, Maria Teresa ; Gargaro, Marco ; Bistoni, Giovanni ; Vacca, Carmine ; Di Michele, Alessandro ; Orabona, Ciriana ; Rosati, Jessica ; Pirro, Matteo ; Giovagnoli, Stefano ; Matino, Davide ; Prontera, Paolo ; Rosi, Gabriella ; Grohmann, Ursula ; Talesa, Vincenzo N. ; Donti, Emilio ; Puccetti, Paolo ; Fallarino, Francesca

Journal of cellular and molecular medicine, 2015-07, Vol.19 (7), p.1593-1605 [Periódico revisado por pares]

England: John Wiley & Sons, Inc

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Título:
Stem cells from human amniotic fluid exert immunoregulatory function via secreted indoleamine 2,3‐dioxygenase1
Autor: Romani, Rita ; Pirisinu, Irene ; Calvitti, Mario ; Pallotta, Maria Teresa ; Gargaro, Marco ; Bistoni, Giovanni ; Vacca, Carmine ; Di Michele, Alessandro ; Orabona, Ciriana ; Rosati, Jessica ; Pirro, Matteo ; Giovagnoli, Stefano ; Matino, Davide ; Prontera, Paolo ; Rosi, Gabriella ; Grohmann, Ursula ; Talesa, Vincenzo N. ; Donti, Emilio ; Puccetti, Paolo ; Fallarino, Francesca
Assuntos: Adult ; Allografts - drug effects ; Amniotic fluid ; Amniotic Fluid - cytology ; Animals ; Biomarkers - metabolism ; CD25 antigen ; CD4 antigen ; Cell Communication - drug effects ; Cell culture ; Cell Differentiation - drug effects ; Cell growth ; Cell proliferation ; Cell Proliferation - drug effects ; Cell Separation ; Cell Shape - drug effects ; Clone Cells ; Cloning ; Dendritic cells ; Dioxygenase ; Embryoid Bodies - cytology ; Fibroblasts ; Fluids ; Foxp3 protein ; Graft Survival - drug effects ; Humans ; Immunomodulation ; Immunomodulation - drug effects ; Immunoregulation ; immunosuppression ; Indoleamine-Pyrrole 2,3,-Dioxygenase - metabolism ; Interferon ; Interferon-gamma - pharmacology ; Leukocytes (mononuclear) ; Lymph nodes ; Lymphocytes ; Lymphocytes T ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, SCID ; Morphology ; Original ; Peripheral blood mononuclear cells ; Phenotype ; Phenotypes ; pluripotent stem cells ; Pluripotent Stem Cells - cytology ; Pluripotent Stem Cells - metabolism ; Proteins ; Skin ; Stem cell transplantation ; Stem cells ; Stem Cells - cytology ; Stem Cells - drug effects ; Stem Cells - immunology ; Stem Cells - metabolism ; T cells ; T-Lymphocytes, Regulatory - drug effects ; T-Lymphocytes, Regulatory - immunology ; Transplantation ; Tumor necrosis factor-TNF ; Vesicles
É parte de: Journal of cellular and molecular medicine, 2015-07, Vol.19 (7), p.1593-1605
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Descrição: Although human amniotic fluid does contain different populations of foetal‐derived stem cells, scanty information is available on the stemness and the potential immunomodulatory activity of in vitro expanded, amniotic fluid stem cells. By means of a methodology unrequiring immune selection, we isolated and characterized different stem cell types from second‐trimester human amniotic fluid samples (human amniotic fluid stem cells, HASCs). Of those populations, one was characterized by a fast doubling time, and cells were thus designated as fHASCs. Cells maintained their original phenotype under prolonged in vitro passaging, and they were able to originate embryoid bodies. Moreover, fHASCs exhibited regulatory properties when treated with interferon (IFN)‐γ, including induction of the immunomodulatory enzyme indoleamine 2,3‐dioxygenase 1 (IDO1). On coculture with human peripheral blood mononuclear cells, IFN‐γ–treated fHASCs caused significantly decreased T‐cell proliferation and increased frequency in CD4+ CD25+ FOXP3+ regulatory T cells. Both effects required an intact IDO1 function and were cell contact‐independent. An unprecedented finding in our study was that purified vesicles from IFN‐γ–treated fHASCs abundantly expressed the functional IDO1 protein, and those vesicles were endowed with an fHASC‐like regulatory function. In vivo, fHASCs were capable of immunoregulatory function, promoting allograft survival in a mouse model of allogeneic skin transplantation. This was concurrent with the expansion of CD4+ CD25+ Foxp3+ T cells in graft‐draining lymph nodes from recipient mice. Thus fHASCs, or vesicles thereof, may represent a novel opportunity for immunoregulatory maneuvers both in vitro and in vivo.
Editor: England: John Wiley & Sons, Inc
Idioma: Inglês

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Stem cells from human amniotic fluid exert immunoregulatory function via secreted indoleamine 2,3‐dioxygenase1

England: John Wiley & Sons, Inc

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