Idioma:

Translesion synthesis mechanisms depend on the nature of DNA damage in UV-irradiated human cells

Quinet, Annabel ; Martins, Davi Jardim ; Vessoni, Alexandre Teixeira ; Biard, Denis ; Sarasin, Alain ; Stary, Anne ; Menck, Carlos Frederico Martins

Nucleic acids research, 2016-07, Vol.44 (12), p.5717-5731 [Periódico revisado por pares]

England: Oxford University Press

Texto completo disponível

Citações Citado por

Enviar para

Título:
Translesion synthesis mechanisms depend on the nature of DNA damage in UV-irradiated human cells
Autor: Quinet, Annabel ; Martins, Davi Jardim ; Vessoni, Alexandre Teixeira ; Biard, Denis ; Sarasin, Alain ; Stary, Anne ; Menck, Carlos Frederico Martins
Assuntos: Adenoviridae - genetics ; Adenoviridae - metabolism ; Cell Line, Transformed ; Deoxyribodipyrimidine Photo-Lyase ; DNA Damage ; DNA Repair ; DNA Replication ; DNA, Single-Stranded - metabolism ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; DNA-Directed DNA Polymerase - genetics ; DNA-Directed DNA Polymerase - metabolism ; Fibroblasts - cytology ; Fibroblasts - metabolism ; Genetic Vectors - chemistry ; Genetic Vectors - metabolism ; Genome Integrity, Repair and ; Genome, Human ; Humans ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; Nucleotidyltransferases - genetics ; Nucleotidyltransferases - metabolism ; Pyrimidine Dimers - metabolism ; S Phase - genetics ; Transduction, Genetic ; Ultraviolet Rays
É parte de: Nucleic acids research, 2016-07, Vol.44 (12), p.5717-5731
Descrição: Ultraviolet-induced 6-4 photoproducts (6-4PP) and cyclobutane pyrimidine dimers (CPD) can be tolerated by translesion DNA polymerases (TLS Pols) at stalled replication forks or by gap-filling. Here, we investigated the involvement of Polη, Rev1 and Rev3L (Polζ catalytic subunit) in the specific bypass of 6-4PP and CPD in repair-deficient XP-C human cells. We combined DNA fiber assay and novel methodologies for detection and quantification of single-stranded DNA (ssDNA) gaps on ongoing replication forks and postreplication repair (PRR) tracts in the human genome. We demonstrated that Rev3L, but not Rev1, is required for postreplicative gap-filling, while Polη and Rev1 are responsible for TLS at stalled replication forks. Moreover, specific photolyases were employed to show that in XP-C cells, CPD arrest replication forks, while 6-4PP are responsible for the generation of ssDNA gaps and PRR tracts. On the other hand, in the absence of Polη or Rev1, both types of lesion block replication forks progression. Altogether, the data directly show that, in the human genome, Polη and Rev1 bypass CPD and 6-4PP at replication forks, while only 6-4PP are also tolerated by a Polζ-dependent gap-filling mechanism, independent of S phase.
Editor: England: Oxford University Press
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Translesion synthesis mechanisms depend on the nature of DNA damage in UV-irradiated human cells

Quinet, Annabel ; Martins, Davi Jardim ; Vessoni, Alexandre Teixeira ; Biard, Denis ; Sarasin, Alain ; Stary, Anne ; Menck, Carlos Frederico Martins

England: Oxford University Press

Buscando em bases de dados remotas. Favor aguardar.