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Physical Non-Viral Gene Delivery Methods for Tissue Engineering

Mellott, Adam J. ; Forrest, M. Laird ; Detamore, Michael S.

Annals of biomedical engineering, 2013-03, Vol.41 (3), p.446-468 [Periódico revisado por pares]

Boston: Springer US

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  • Título:
    Physical Non-Viral Gene Delivery Methods for Tissue Engineering
  • Autor: Mellott, Adam J. ; Forrest, M. Laird ; Detamore, Michael S.
  • Assuntos: Animals ; Biochemistry ; Biological and Medical Physics ; Biomedical and Life Sciences ; Biomedical Engineering ; Biomedical Engineering and Bioengineering ; Biomedicine ; Biophysics ; Classical Mechanics ; DNA - administration & dosage ; DNA - genetics ; Gene Transfer Techniques - trends ; Genetic Therapy ; Genetic Vectors ; Humans ; Tissue Engineering - methods ; Tissue Engineering - trends
  • É parte de: Annals of biomedical engineering, 2013-03, Vol.41 (3), p.446-468
  • Notas: ObjectType-Article-2
    SourceType-Scholarly Journals-1
    ObjectType-Feature-3
    content type line 23
    ObjectType-Review-1
    ObjectType-Article-1
    ObjectType-Feature-2
  • Descrição: The integration of gene therapy into tissue engineering to control differentiation and direct tissue formation is not a new concept; however, successful delivery of nucleic acids into primary cells, progenitor cells, and stem cells has proven exceptionally challenging. Viral vectors are generally highly effective at delivering nucleic acids to a variety of cell populations, both dividing and non-dividing, yet these viral vectors are marred by significant safety concerns. Non-viral vectors are preferred for gene therapy, despite lower transfection efficiencies, and possess many customizable attributes that are desirable for tissue engineering applications. However, there is no single non-viral gene delivery strategy that “fits-all” cell types and tissues. Thus, there is a compelling opportunity to examine different non-viral vectors, especially physical vectors, and compare their relative degrees of success. This review examines the advantages and disadvantages of physical non-viral methods (i.e., microinjection, ballistic gene delivery, electroporation, sonoporation, laser irradiation, magnetofection, and electric field-induced molecular vibration), with particular attention given to electroporation because of its versatility, with further special emphasis on Nucleofection™. In addition, attributes of cellular character that can be used to improve differentiation strategies are examined for tissue engineering applications. Ultimately, electroporation exhibits a high transfection efficiency in many cell types, which is highly desirable for tissue engineering applications, but electroporation and other physical non-viral gene delivery methods are still limited by poor cell viability. Overcoming the challenge of poor cell viability in highly efficient physical non-viral techniques is the key to using gene delivery to enhance tissue engineering applications.
  • Editor: Boston: Springer US
  • Idioma: Inglês
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