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Generation of High-Amylose Rice through CRISPR/Cas9-Mediated Targeted Mutagenesis of Starch Branching Enzymes

Sun, Yongwei ; Jiao, Guiai ; Liu, Zupei ; Zhang, Xin ; Li, Jingying ; Guo, Xiuping ; Du, Wenming ; Du, Jinlu ; Francis, Frédéric ; Zhao, Yunde ; Xia, Lanqin

Frontiers in plant science, 2017-03, Vol.8, p.298 [Periódico revisado por pares]

Switzerland: Frontiers Research Foundation

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  • Título:
    Generation of High-Amylose Rice through CRISPR/Cas9-Mediated Targeted Mutagenesis of Starch Branching Enzymes
  • Autor: Sun, Yongwei ; Jiao, Guiai ; Liu, Zupei ; Zhang, Xin ; Li, Jingying ; Guo, Xiuping ; Du, Wenming ; Du, Jinlu ; Francis, Frédéric ; Zhao, Yunde ; Xia, Lanqin
  • Assuntos: amylose content (AC) ; Biotechnologie ; Biotechnology ; branching enzyme (SBE) ; CRISPR/Cas9 ; genome editing ; Life sciences ; Plant Science ; resistant starch (RS) ; rice ; Sciences du vivant ; starch
  • É parte de: Frontiers in plant science, 2017-03, Vol.8, p.298
  • Notas: scopus-id:2-s2.0-85014906772
    Edited by: Agnieszka Ludwików, Adam Mickiewicz University in Poznań, Poland
    This article was submitted to Plant Biotechnology, a section of the journal Frontiers in Plant Science
    Reviewed by: Ibrokhim Abdurakhmonov, The Center of Genomics and Bioinformatics, Uzbekistan; Yuriko Osakabe, University of Tokushima, Japan
    These authors have contributed equally to this work.
  • Descrição: Cereals high in amylose content (AC) and resistant starch (RS) offer potential health benefits. Previous studies using chemical mutagenesis or RNA interference have demonstrated that starch branching enzyme (SBE) plays a major role in determining the fine structure and physical properties of starch. However, it remains a challenge to control starch branching in commercial lines. Here, we use CRISPR/Cas9 technology to generate targeted mutagenesis in and in rice. The frequencies of obtained homozygous or bi-allelic mutant lines with indels in and in T generation were from 26.7 to 40%. Mutations in the homozygous T lines stably transmitted to the T generation and those in the bi-allelic lines segregated in a Mendelian fashion. Transgene-free plants carrying only the frame-shifted mutagenesis were recovered in T generation following segregation. Whereas no obvious differences were observed between the mutants and wild type, mutants showed higher proportion of long chains presented in debranched amylopectin, significantly increased AC and RS content to as higher as 25.0 and 9.8%, respectively, and thus altered fine structure and nutritional properties of starch. Taken together, our results demonstrated for the first time the feasibility to create high-amylose rice through CRISPR/Cas9-mediated editing of .
  • Editor: Switzerland: Frontiers Research Foundation
  • Idioma: Inglês
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