Idioma:

PML protein organizes heterochromatin domains where it regulates histone H3.3 deposition by ATRX/DAXX

Delbarre, Erwan ; Ivanauskiene, Kristina ; Spirkoski, Jane ; Shah, Akshay ; Vekterud, Kristin ; Moskaug, Jan Øivind ; Bøe, Stig Ove ; Wong, Lee H ; Küntziger, Thomas ; Collas, Philippe

Genome research, 2017-06, Vol.27 (6), p.913-921 [Periódico revisado por pares]

United States: Cold Spring Harbor Laboratory Press

Texto completo disponível

Citações Citado por

Enviar para

Título:
PML protein organizes heterochromatin domains where it regulates histone H3.3 deposition by ATRX/DAXX
Autor: Delbarre, Erwan ; Ivanauskiene, Kristina ; Spirkoski, Jane ; Shah, Akshay ; Vekterud, Kristin ; Moskaug, Jan Øivind ; Bøe, Stig Ove ; Wong, Lee H ; Küntziger, Thomas ; Collas, Philippe
Assuntos: Animals ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Chromatin Assembly and Disassembly ; Fibroblasts - cytology ; Fibroblasts - metabolism ; Fluorescence Recovery After Photobleaching ; Gene Expression Regulation ; Heterochromatin - metabolism ; Heterochromatin - ultrastructure ; Histones - genetics ; Histones - metabolism ; Intracellular Signaling Peptides and Proteins - genetics ; Intracellular Signaling Peptides and Proteins - metabolism ; Methylation ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; Nucleosomes - metabolism ; Nucleosomes - ultrastructure ; Promyelocytic Leukemia Protein - genetics ; Promyelocytic Leukemia Protein - metabolism ; Signal Transduction ; X-linked Nuclear Protein - genetics ; X-linked Nuclear Protein - metabolism
É parte de: Genome research, 2017-06, Vol.27 (6), p.913-921
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
NFR/249734
These authors contributed equally to this work.
Descrição: Maintenance of chromatin homeostasis involves proper delivery of histone variants to the genome. The interplay between different chaperones regulating the supply of histone variants to distinct chromatin domains remains largely undeciphered. We report a role of promyelocytic leukemia (PML) protein in the routing of histone variant H3.3 to chromatin and in the organization of megabase-size heterochromatic PML-associated domains that we call PADs. Loss of PML alters the heterochromatic state of PADs by shifting the histone H3 methylation balance from K9me3 to K27me3. Loss of PML impairs deposition of H3.3 by ATRX and DAXX in PADs but preserves the H3.3 loading function of HIRA in these regions. Our results unveil an unappreciated role of PML in the large-scale organization of chromatin and demonstrate a PML-dependent role of ATRX/DAXX in the deposition of H3.3 in PADs. Our data suggest that H3.3 loading by HIRA and ATRX-dependent H3K27 trimethylation constitute mechanisms ensuring maintenance of heterochromatin when the integrity of these domains is compromised.
Editor: United States: Cold Spring Harbor Laboratory Press
Idioma: Inglês;Norueguês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 7 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

PML protein organizes heterochromatin domains where it regulates histone H3.3 deposition by ATRX/DAXX

Delbarre, Erwan ; Ivanauskiene, Kristina ; Spirkoski, Jane ; Shah, Akshay ; Vekterud, Kristin ; Moskaug, Jan Øivind ; Bøe, Stig Ove ; Wong, Lee H ; Küntziger, Thomas ; Collas, Philippe

United States: Cold Spring Harbor Laboratory Press

Buscando em bases de dados remotas. Favor aguardar.