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Mechanosensing is critical for axon growth in the developing brain

Koser, David E ; Thompson, Amelia J ; Foster, Sarah K ; Dwivedy, Asha ; Pillai, Eva K ; Sheridan, Graham K ; Svoboda, Hanno ; Viana, Matheus ; Costa, Luciano da F ; Guck, Jochen ; Holt, Christine E ; Franze, Kristian

Nature neuroscience, 2016-12, Vol.19 (12), p.1592-1598 [Periódico revisado por pares]

United States: Nature Publishing Group

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Título:
Mechanosensing is critical for axon growth in the developing brain
Autor: Koser, David E ; Thompson, Amelia J ; Foster, Sarah K ; Dwivedy, Asha ; Pillai, Eva K ; Sheridan, Graham K ; Svoboda, Hanno ; Viana, Matheus ; Costa, Luciano da F ; Guck, Jochen ; Holt, Christine E ; Franze, Kristian
Assuntos: Animals ; Axons - metabolism ; Axons - pathology ; Brain - growth & development ; Mechanical properties ; Mechanotransduction, Cellular - physiology ; Microscopy ; Neurogenesis - physiology ; Neurons ; Neurosciences ; Retina - metabolism ; Retinal Ganglion Cells ; Visual Pathways - growth & development ; Xenopus ; Xenopus laevis ; Zebrafish
É parte de: Nature neuroscience, 2016-12, Vol.19 (12), p.1592-1598
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Present address: Biotechnology Center, Technische Universität Dresden, Dresden, Germany
Present address: School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK
Present address: Department of Clinical Neurobiology, Medical Faculty of Heidelberg University and German Cancer Research Center (DKFZ), Heidelberg, Germany
Descrição: During nervous system development, neurons extend axons along well-defined pathways. The current understanding of axon pathfinding is based mainly on chemical signaling. However, growing neurons interact not only chemically but also mechanically with their environment. Here we identify mechanical signals as important regulators of axon pathfinding. In vitro, substrate stiffness determined growth patterns of Xenopus retinal ganglion cell axons. In vivo atomic force microscopy revealed a noticeable pattern of stiffness gradients in the embryonic brain. Retinal ganglion cell axons grew toward softer tissue, which was reproduced in vitro in the absence of chemical gradients. To test the importance of mechanical signals for axon growth in vivo, we altered brain stiffness, blocked mechanotransduction pharmacologically and knocked down the mechanosensitive ion channel piezo1. All treatments resulted in aberrant axonal growth and pathfinding errors, suggesting that local tissue stiffness, read out by mechanosensitive ion channels, is critically involved in instructing neuronal growth in vivo.
Editor: United States: Nature Publishing Group
Idioma: Inglês

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Mechanosensing is critical for axon growth in the developing brain

Koser, David E ; Thompson, Amelia J ; Foster, Sarah K ; Dwivedy, Asha ; Pillai, Eva K ; Sheridan, Graham K ; Svoboda, Hanno ; Viana, Matheus ; Costa, Luciano da F ; Guck, Jochen ; Holt, Christine E ; Franze, Kristian

United States: Nature Publishing Group

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