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Nitrate Reductase Knockout Uncouples Nitrate Transport from Nitrate Assimilation and Drives Repartitioning of Carbon Flux in a Model Pennate Diatom

McCarthy, James K. ; Smith, Sarah R. ; McCrow, John P. ; Tan, Maxine ; Zheng, Hong ; Beeri, Karen ; Roth, Robyn ; Lichtle, Christian ; Goodenough, Ursula ; Bowler, Chris P. ; Dupont, Christopher L. ; Allen, Andrew E.

The Plant cell, 2017-08, Vol.29 (8), p.2047-2070 [Periódico revisado por pares]

England: American Society of Plant Biologists

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Título:
Nitrate Reductase Knockout Uncouples Nitrate Transport from Nitrate Assimilation and Drives Repartitioning of Carbon Flux in a Model Pennate Diatom
Autor: McCarthy, James K. ; Smith, Sarah R. ; McCrow, John P. ; Tan, Maxine ; Zheng, Hong ; Beeri, Karen ; Roth, Robyn ; Lichtle, Christian ; Goodenough, Ursula ; Bowler, Chris P. ; Dupont, Christopher L. ; Allen, Andrew E.
Assuntos: Assimilation ; BASIC BIOLOGICAL SCIENCES ; Biochemical composition ; Biological Transport - drug effects ; Biosynthesis ; Biosynthetic Pathways - genetics ; Carbon ; Carbon - metabolism ; Carbon Cycle - drug effects ; Carbon fixation ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Chlorophyll ; Chlorophyll - biosynthesis ; Deactivation ; Diatoms - enzymology ; Diatoms - metabolism ; Diatoms - physiology ; Diatoms - ultrastructure ; Ecological effects ; Electron micrographs ; Esters - metabolism ; Gene expression ; Gene Expression Regulation - drug effects ; Gene Knockout Techniques ; Genes ; Inactivation ; Lipid Metabolism - drug effects ; Lipid Metabolism - genetics ; Marine environment ; Metabolism ; Nitrate reductase ; Nitrate Reductase - metabolism ; Nitrates ; Nitrates - metabolism ; Nitrates - pharmacology ; Photosynthesis - drug effects ; Photosystem ; Protein Biosynthesis - drug effects ; Proteins ; Storage ; Thylakoids - drug effects ; Thylakoids - metabolism ; Transcription activation ; Transcription, Genetic - drug effects ; Transcriptome - genetics ; Transport ; Triglycerides ; Triglycerides - metabolism ; Vacuoles ; Vacuoles - drug effects ; Vacuoles - metabolism
É parte de: The Plant cell, 2017-08, Vol.29 (8), p.2047-2070
Notas: National Science Foundation (NSF)
SC00006719; SC0008593; SC0006719; MCB-1024913; GBMF3828
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Gordon and Betty Moore Foundation (United States)
www.plantcell.org/cgi/doi/10.1105/tpc.16.00910
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Andrew E. Allen (aallen@jcvi.org).
Descrição: The ecological prominence of diatoms in the ocean environment largely results from their superior competitive ability for dissolved nitrate (NO3 −). To investigate the cellular and genetic basis of diatom NO3 − assimilation, we generated a knockout in the nitrate reductase gene (NR-KO) of the model pennate diatom Phaeodactylum tricornutum. In NR-KO cells, N-assimilation was abolished although NO3 − transport remained intact. Unassimilated NO3 − accumulated in NR-KO cells, resulting in swelling and associated changes in biochemical composition and physiology. Elevated expression of genes encoding putative vacuolar NO3 − chloride channel transporters plus electron micrographs indicating enlarged vacuoles suggested vacuolar storage of NO3 −. Triacylglycerol concentrations in the NR-KO cells increased immediately following the addition of NO3 −, and these increases coincided with elevated gene expression of key triacylglycerol biosynthesis components. Simultaneously, induction of transcripts encoding proteins involved in thylakoid membrane lipid recycling suggested more abrupt repartitioning of carbon resources in NR-KO cells compared with the wild type. Conversely, ribosomal structure and photosystem genes were immediately deactivated in NR-KO cells following NO3 − addition, followed within hours by deactivation of genes encoding enzymes for chlorophyll biosynthesis and carbon fixation and metabolism. N-assimilation pathway genes respond uniquely, apparently induced simultaneously by both NO3 − replete and deplete conditions.
Editor: England: American Society of Plant Biologists
Idioma: Inglês

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Nitrate Reductase Knockout Uncouples Nitrate Transport from Nitrate Assimilation and Drives Repartitioning of Carbon Flux in a Model Pennate Diatom

McCarthy, James K. ; Smith, Sarah R. ; McCrow, John P. ; Tan, Maxine ; Zheng, Hong ; Beeri, Karen ; Roth, Robyn ; Lichtle, Christian ; Goodenough, Ursula ; Bowler, Chris P. ; Dupont, Christopher L. ; Allen, Andrew E.

England: American Society of Plant Biologists

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