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Genetic and Transcriptomic Analyses of Ciprofloxacin-Tolerant Staphylococcus aureus Isolated by the Replica Plating Tolerance Isolation System (REPTIS)

Matsuo, Miki ; Hiramatsu, Miyu ; Singh, Madhuri ; Sasaki, Takashi ; Hishinuma, Tomomi ; Yamamoto, Norio ; Morimoto, Yuh ; Kirikae, Teruo ; Hiramatsu, Keiichi

Antimicrobial agents and chemotherapy, 2019-02, Vol.63 (2) [Periódico revisado por pares]

United States: American Society for Microbiology

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Título:
Genetic and Transcriptomic Analyses of Ciprofloxacin-Tolerant Staphylococcus aureus Isolated by the Replica Plating Tolerance Isolation System (REPTIS)
Autor: Matsuo, Miki ; Hiramatsu, Miyu ; Singh, Madhuri ; Sasaki, Takashi ; Hishinuma, Tomomi ; Yamamoto, Norio ; Morimoto, Yuh ; Kirikae, Teruo ; Hiramatsu, Keiichi
Assuntos: Anti-Bacterial Agents ; Anti-Bacterial Agents - pharmacology ; Ciprofloxacin ; Ciprofloxacin - pharmacology ; Drug Resistance, Bacterial ; Drug Resistance, Bacterial - genetics ; Editor's Pick ; Gene Expression Profiling - methods ; GTP Pyrophosphokinase - genetics ; Humans ; Leucine-tRNA Ligase - genetics ; Mechanisms of Resistance ; Microbial Sensitivity Tests ; Microbial Sensitivity Tests - methods ; Mupirocin - pharmacology ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - genetics ; Staphylococcus aureus - isolation & purification
É parte de: Antimicrobial agents and chemotherapy, 2019-02, Vol.63 (2)
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Citation Matsuo M, Hiramatsu M, Singh M, Sasaki T, Hishinuma T, Yamamoto N, Morimoto Y, Kirikae T, Hiramatsu K. 2019. Genetic and transcriptomic analyses of ciprofloxacin-tolerant Staphylococcus aureus isolated by the replica plating tolerance isolation system (REPTIS). Antimicrob Agents Chemother 63:e02019-18. https://doi.org/10.1128/AAC.02019-18.
Descrição: We developed a simple, efficient, and cost-effective method, named the plica lating olerance solation ystem (REPTIS), to detect the antibiotic tolerance potential of a bacterial strain. This method can also be used to quantify the antibiotic-tolerant subpopulation in a susceptible population. Using REPTIS, we isolated ciprofloxacin (CPFX)-tolerant mutants (mutants R2, R3, R5, and R6) carrying a total of 12 mutations in 12 different genes from methicillin-sensitive (MSSA) strain FDA209P. Each mutant carried multiple mutations, while few strains shared the same mutation. The R2 strain carried a nonsense mutation in the stress-mediating gene, Additionally, two strains carried the same point mutation in the gene, encoding leucyl-tRNA synthetase. Furthermore, RNA sequencing of the R strains showed a common upregulation of Overall, transcriptome analysis showed downregulation of genes related to translation; carbohydrate, fat, and energy metabolism; nucleotide synthesis; and upregulation of amino acid biosynthesis and transportation genes in R2, R3, and R6, similar to the findings observed for the FDA209P strain treated with mupirocin (MUP0.03). However, R5 showed a unique transcription pattern that differed from that of MUP0.03. REPTIS is a unique and convenient method for quantifying the level of tolerance of a clinical isolate. Genomic and transcriptomic analyses of R strains demonstrated that CPFX tolerance in these mutants occurs via at least two distinct mechanisms, one of which is similar to that which occurs with mupirocin treatment.
Editor: United States: American Society for Microbiology
Idioma: Inglês

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Genetic and Transcriptomic Analyses of Ciprofloxacin-Tolerant Staphylococcus aureus Isolated by the Replica Plating Tolerance Isolation System (REPTIS)

Matsuo, Miki ; Hiramatsu, Miyu ; Singh, Madhuri ; Sasaki, Takashi ; Hishinuma, Tomomi ; Yamamoto, Norio ; Morimoto, Yuh ; Kirikae, Teruo ; Hiramatsu, Keiichi

United States: American Society for Microbiology

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