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Cu Transport by the Extended Family of CcoA-like Transporters (CalT) in Proteobacteria

Zhang, Yang ; Blaby-Haas, Crysten E ; Steimle, Stefan ; Verissimo, Andreia F ; Garcia-Angulo, Victor A ; Koch, Hans-Georg ; Daldal, Fevzi ; Khalfaoui-Hassani, Bahia

Scientific reports, 2019-02, Vol.9 (1), p.1208-1208, Article 1208 [Periódico revisado por pares]

England: Nature Publishing Group

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Título:
Cu Transport by the Extended Family of CcoA-like Transporters (CalT) in Proteobacteria
Autor: Zhang, Yang ; Blaby-Haas, Crysten E ; Steimle, Stefan ; Verissimo, Andreia F ; Garcia-Angulo, Victor A ; Koch, Hans-Georg ; Daldal, Fevzi ; Khalfaoui-Hassani, Bahia
Assuntos: Bacterial Proteins - metabolism ; Bacteriology ; BASIC BIOLOGICAL SCIENCES ; Biochemistry, Molecular Biology ; Biological Transport ; Carrier Proteins - metabolism ; cbb3 ; CcoA and RfnT ; Copper ; Copper - metabolism ; Copper transport ; cytochrome coxidase ; Cytochrome-c oxidase ; Cytochromes c - metabolism ; Electron Transport Complex IV - genetics ; Genomics ; Life Sciences ; Membrane Transport Proteins - metabolism ; MFS-type transporter ; Microbiology and Parasitology ; Mutants ; Proteins ; Proteobacteria - metabolism ; Rhizobium leguminosarum - genetics ; Rhodobacter capsulatus - genetics ; Riboflavin ; Riboflavin - metabolism ; Riboflavin transport ; Vitamin B
É parte de: Scientific reports, 2019-02, Vol.9 (1), p.1208-1208, Article 1208
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
SC0012704; FG02-91ER20052
BNL-211298-2019-JAAM
USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Descrição: Comparative genomic studies of the bacterial MFS-type copper importer CcoA, required for cbb -type cytochrome c oxidase (cbb -Cox) biogenesis, revealed a widespread CcoA-like transporters (CalT) family, containing the conserved CcoA Cu-binding MxxxM and HxxxM motifs. Surprisingly, this family also included the RfnT-like proteins, earlier suggested to transport riboflavin. However, presence of the Cu-binding motifs in these proteins raised the possibility that they might be Cu transporters. To test this hypothesis, the genomic context of the corresponding genes was examined, and three of such genes from Ochrobactrum anthropi, Rhodopseudomonas palustris and Agrobacterium tumefaciens were expressed in Escherichia coli (ΔribB) and Rhodobacter capsulatus (ΔccoA) mutants. Copper and riboflavin uptake abilities of these strains were compared with those expressing R. capsulatus CcoA and Rhizobium leguminosarum RibN as bona fide copper and riboflavin importers, respectively. Overall data demonstrated that the "RfnT-like" CalT proteins are unable to efficiently transport riboflavin, but they import copper like CcoA. Nevertheless, even though expressed and membrane-localized in a R. capsulatus mutant lacking CcoA, these transporters were unable to accumulate Cu or complement for cbb -Cox defect. This lack of functional exchangeability between the different subfamilies of CalT homologs suggests that MFS-type bacterial copper importers might be species-specific.
Editor: England: Nature Publishing Group
Idioma: Inglês

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Cu Transport by the Extended Family of CcoA-like Transporters (CalT) in Proteobacteria

Zhang, Yang ; Blaby-Haas, Crysten E ; Steimle, Stefan ; Verissimo, Andreia F ; Garcia-Angulo, Victor A ; Koch, Hans-Georg ; Daldal, Fevzi ; Khalfaoui-Hassani, Bahia

England: Nature Publishing Group

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