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A plant DNA virus replicates in the salivary glands of its insect vector via recruitment of host DNA synthesis machinery

He, Ya-Zhou ; Wang, Yu-Meng ; Yin, Tian-Yan ; Fiallo-Olivé, Elvira ; Liu, Yin-Quan ; Hanley-Bowdoin, Linda ; Wang, Xiao-Wei

Proceedings of the National Academy of Sciences - PNAS, 2020-07, Vol.117 (29), p.16928-16937 [Periódico revisado por pares]

Washington: National Academy of Sciences

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  • Título:
    A plant DNA virus replicates in the salivary glands of its insect vector via recruitment of host DNA synthesis machinery
  • Autor: He, Ya-Zhou ; Wang, Yu-Meng ; Yin, Tian-Yan ; Fiallo-Olivé, Elvira ; Liu, Yin-Quan ; Hanley-Bowdoin, Linda ; Wang, Xiao-Wei
  • Assuntos: Antigens ; Biological Sciences ; Deoxyribonucleic acid ; DNA ; DNA biosynthesis ; DNA polymerase ; DNA viruses ; DNA-directed DNA polymerase ; Host plants ; Insects ; Leaf-curl ; Leaves ; Machinery ; Plant diseases ; Plant viruses ; PNAS Plus ; Proliferating cell nuclear antigen ; Replication ; RNA viruses ; Salivary gland ; Salivary glands ; Single-stranded DNA ; Tomatoes ; Vectors ; Viruses ; Yellow leaf
  • É parte de: Proceedings of the National Academy of Sciences - PNAS, 2020-07, Vol.117 (29), p.16928-16937
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
    Edited by George E. Bruening, University of California, Davis, CA, and approved June 3, 2020 (received for review November 27, 2018)
    Author contributions: Y.-Z.H., Y.-Q.L., and X.-W.W. designed research; Y.-Z.H., Y.-M.W., and T.-Y.Y. performed research; Y.-Z.H., Y.-M.W., E.F.-O., Y.-Q.L., L.H.-B., and X.-W.W. analyzed data; and Y.-Z.H., Y.-M.W., E.F.-O., L.H.-B., and X.-W.W. wrote the paper.
    1Y.-Z.H. and Y.-M.W. contributed equally to this work.
  • Descrição: Whereas most of the arthropod-borne animal viruses replicate in their vectors, this is less common for plant viruses. So far, only some plant RNA viruses have been demonstrated to replicate in insect vectors and plant hosts. How plant viruses evolved to replicate in the animal kingdom remains largely unknown. Geminiviruses comprise a large family of plant-infecting, single-stranded DNA viruses that cause serious crop losses worldwide. Here, we report evidence and insight into the replication of the geminivirus tomato yellow leaf curl virus (TYLCV) in the whitefly (Bemisia tabaci) vector and that replication is mainly in the salivary glands. We found that TYLCV induces DNA synthesis machinery, proliferating cell nuclear antigen (PCNA) and DNA polymerase δ (Polδ), to establish a replication-competent environment in whiteflies. TYLCV replication-associated protein (Rep) interacts with whitefly PCNA, which recruits DNA Polδ for virus replication. In contrast, another geminivirus, papaya leaf curl China virus (PaLCuCNV), does not replicate in the whitefly vector. PaLCuCNV does not induce DNA-synthesis machinery, and the Rep does not interact with whitefly PCNA. Our findings reveal important mechanisms by which a plant DNA virus replicates across the kingdom barrier in an insect and may help to explain the global spread of this devastating pathogen.
  • Editor: Washington: National Academy of Sciences
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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