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A Machine Learning Study on the Thermostability Prediction of (R)-ω-Selective Amine Transaminase from Aspergillus terreus

Jia, Li-li ; Sun, Ting-ting ; Wang, Yan ; Shen, Yu McClellan, David A. ; David A McClellan

BioMed research international, 2021, Vol.2021, p.1-7 [Periódico revisado por pares]

New York: Hindawi

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Título:
A Machine Learning Study on the Thermostability Prediction of (R)-ω-Selective Amine Transaminase from Aspergillus terreus
Autor: Jia, Li-li ; Sun, Ting-ting ; Wang, Yan ; Shen, Yu
McClellan, David A. ; David A McClellan
Assuntos: Algorithms ; Amino acid sequence ; Amino acids ; Artificial intelligence ; Aspergillus terreus ; Biocatalysts ; Biotechnology ; Catalysis ; Catalytic activity ; Computer simulation ; Computers ; Digital signal processing ; Efficiency ; Engineering ; Enzymes ; Evolution ; Experimental data ; Fast Fourier transformations ; Fourier transforms ; Industrial production ; Learning algorithms ; Least squares method ; Machine learning ; Mutagenesis ; Mutants ; Mutation ; Predictions ; Protein engineering ; Protein structure ; Proteins ; Screening ; Signal processing ; Stability analysis ; Structure-function relationships ; Thermal stability ; Transaminase ; Transaminases
É parte de: BioMed research international, 2021, Vol.2021, p.1-7
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Academic Editor: David A. McClellan
Descrição: Artificial intelligence technologies such as machine learning have been applied to protein engineering, with unique advantages in protein structure, function prediction, catalytic activity, and other issues in recent years. Screening better mutants is still a bottleneck in protein engineering. In this paper, a new sequence-activity relationship method was analyzed for its application in improving the thermal stability of Aspergillus terreus (R)-ω-selective amine transaminase. The experimental data from 6 single-point mutated enzymes were used as a learning dataset to build models and predict the thermostability of 26 mutants. Based on digital signal processing (DSP), this method digitized the amino acid sequence of proteins by fast Fourier transform (FFT) and then established the best model applying partial least squares regression (PLSR) to screen out all possible mutants, especially those with high performance. In protein engineering, the innovative sequence activity relationship (ISAR) method can make a reasonable prediction using limited experimental data and significantly reduce the experimental cost. The half-life (T1/2) of (R)-ω-transaminase was fitted with the amino acid sequence by the ISAR algorithm, resulting in an R2 of 0.8929 and a cvRMSE of 4.89. At the same time, the mutants with higher T1/2 than the existing ones were predicted, laying the groundwork for better (R)-ω-transaminase in the later stage. The ISAR algorithm is expected to provide a new technique for protein evolution and screening.
Editor: New York: Hindawi
Idioma: Inglês

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A Machine Learning Study on the Thermostability Prediction of (R)-ω-Selective Amine Transaminase from Aspergillus terreus

Jia, Li-li ; Sun, Ting-ting ; Wang, Yan ; Shen, Yu McClellan, David A. ; David A McClellan

New York: Hindawi

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