Idioma:

Establishment and characterization of urothelial carcinoma cell lines with and without BRAF mutation (V595E) in dogs

Yamasaki, Hirofumi ; Uematsu, Yosuke ; Okano, Kumiko ; Ichikawa, Mika ; Tei, Meina ; Hirabayashi, Miyuki ; Uchida, Kazuyuki ; Ono, Kenichiro ; Hirao, Hidehiro

In vitro cellular & developmental biology. Animal, 2022-12, Vol.58 (10), p.898-911 [Periódico revisado por pares]

New York: Springer US

Texto completo disponível

Citações Citado por

Enviar para

Título:
Establishment and characterization of urothelial carcinoma cell lines with and without BRAF mutation (V595E) in dogs
Autor: Yamasaki, Hirofumi ; Uematsu, Yosuke ; Okano, Kumiko ; Ichikawa, Mika ; Tei, Meina ; Hirabayashi, Miyuki ; Uchida, Kazuyuki ; Ono, Kenichiro ; Hirao, Hidehiro
Assuntos: Animal Genetics and Genomics ; Animals ; Biomedical and Life Sciences ; Bladder cancer ; Carcinoma, Transitional Cell - genetics ; Carcinoma, Transitional Cell - pathology ; Carcinoma, Transitional Cell - veterinary ; Cell Biology ; Cell Culture ; Cell Line - cytology ; Cell Line - metabolism ; Cell Line, Tumor - cytology ; Cell Line, Tumor - metabolism ; Cell proliferation ; Cloning ; Developmental Biology ; Dog Diseases - genetics ; Dog Diseases - metabolism ; Dogs ; Extracellular signal-regulated kinase ; Kinases ; Life Sciences ; MAP kinase ; Mice ; Mutation ; Mutation - genetics ; Phosphors ; Point mutation ; Protein kinase ; Proto-Oncogene Proteins B-raf - genetics ; Raf protein ; Sarcoma ; Severe combined immunodeficiency ; Signal transduction ; Signaling ; Stem Cells ; Tumor cell lines ; Tumor cells ; Tumorigenicity ; Tumors ; Urinary Bladder Neoplasms - genetics ; Urinary Bladder Neoplasms - pathology ; Urinary Bladder Neoplasms - veterinary ; Urothelial carcinoma ; Viruses ; Xenografts
É parte de: In vitro cellular & developmental biology. Animal, 2022-12, Vol.58 (10), p.898-911
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Each 5 urothelial carcinoma (UC) cell lines with and without the v-Raf murine sarcoma virus oncogene homolog B (BRAF) gene mutation (V595E) were established and examined V595E-related tumorigenic characteristics in dogs. No typical morphological features were observed in cloned cells with and without V595E. The cell proliferation of both cloned cells showed logarithmic growth curve and those doubling time were 24.9 ± 4.1 h in V595E ( +) and 29.3 ± 11.3 h in V595E ( −). On the growth curve of xenotransplanted tumor in severe combined immunodeficiency mice, 3 out of 5 V595E ( +) and 2 out of 5 V595E ( −) cloned cells revealed gradually and remarkably increasing curve, indicating clearly tumorigenicity. The xenotransplanted tumors with V595E ( +) showed typical features of UC, such as solid proliferation of pleomorphic tumor cells, formation of papillary structure, and glandular structure. Additionally, various vascular formation was observed, probably indicating an advanced growth phase of UC. In mitogen-activated protein kinase (MAPK) signaling pathway, cytoplasmic phosphorylated-BRAF (pBRAF) and cytoplasmic and nuclear phosphorylated-ERK1/2 (pERK1/2) were detected in all 4 tumors with V595E ( +), whereas only cytoplasmic and nuclear pERK1/2 was detected in tumors with V595E ( −). Since V595E can directly activate MAPK signaling pathway, coincidence of V595E with pBRAF (phosphor Thr598/Ser601) indicates acquired resistance to BRAF inhibitors. These established UC cell lines, especially V595E ( +) cell lines, are useful tool for understanding pathophysiological states and controlling therapeutic manners of UC in dogs.
Editor: New York: Springer US
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Establishment and characterization of urothelial carcinoma cell lines with and without BRAF mutation (V595E) in dogs

Yamasaki, Hirofumi ; Uematsu, Yosuke ; Okano, Kumiko ; Ichikawa, Mika ; Tei, Meina ; Hirabayashi, Miyuki ; Uchida, Kazuyuki ; Ono, Kenichiro ; Hirao, Hidehiro

New York: Springer US

Buscando em bases de dados remotas. Favor aguardar.