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Cellular Distribution Pattern of tjp1 (ZO-1) in Xenopus laevis Oocytes Heterologously Expressing Claudins

Brunner, Nora ; Stein, Laura ; Amasheh, Salah

The Journal of membrane biology, 2023-02, Vol.256 (1), p.51-61 [Periódico revisado por pares]

New York: Springer US

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Título:
Cellular Distribution Pattern of tjp1 (ZO-1) in Xenopus laevis Oocytes Heterologously Expressing Claudins
Autor: Brunner, Nora ; Stein, Laura ; Amasheh, Salah
Assuntos: Animals ; Biochemistry ; Biomedical and Life Sciences ; Claudin-1 - genetics ; Claudin-1 - metabolism ; Claudins - genetics ; Claudins - metabolism ; Cytoskeleton ; Effectors ; Gametocytes ; Gene expression ; Human Physiology ; Humans ; Life Sciences ; mRNA ; Oocytes ; Oocytes - metabolism ; Protein interaction ; Proteins ; Scaffolding ; Tight Junctions - metabolism ; Xenopus laevis ; Xenopus laevis - metabolism ; Zonula occludens-1 protein ; Zonula Occludens-1 Protein - genetics ; Zonula Occludens-1 Protein - metabolism
É parte de: The Journal of membrane biology, 2023-02, Vol.256 (1), p.51-61
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Epithelial barriers constitute a fundamental requirement in every organism, as they allow the separation of different environments and set boundaries against noxious and other adverse effectors. In many inflammatory and degenerative diseases, epithelial barrier function is impaired because of a disturbance of the paracellular seal. Recently, the Xenopus laevis oocyte has been established as a heterologous expression model for the analysis of transmembrane tight junction protein interactions and is currently considered to be a suitable screening model for barrier effectors. A prerequisite for this application is a physiological anchoring of claudins to the cytoskeleton via the major scaffolding protein tjp1 (tight junction protein 1, ZO-1). We have analyzed the oocyte model with regard to the interaction of heterologously expressed claudins and tjp1. Our experiments have revealed endogenous tjp1 expression in protein and mRNA analyses of unfertilized Xenopus laevis oocytes expressing human claudin 1 (CLDN1) to claudin 5 (CLDN5). The amphibian cell model can therefore be used for the analysis of claudin interactions. Graphical Abstract
Editor: New York: Springer US
Idioma: Inglês

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Cellular Distribution Pattern of tjp1 (ZO-1) in Xenopus laevis Oocytes Heterologously Expressing Claudins

Brunner, Nora ; Stein, Laura ; Amasheh, Salah

New York: Springer US

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