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Method Validation for Simultaneous Determination of Cholesterol and Cholesterol Oxides in Milk by RP-HPLC-DAD

Bauer, Luciana C. ; Santana, Débora de A. ; Macedo, Marianne dos S. ; Torres, Alexandre G. ; Souza, Nilson E. de ; Simionato, Julliana I.

Journal of the Brazilian Chemical Society, 2014-01, Vol.25 (1), p.161-168 [Periódico revisado por pares]

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Título:
Method Validation for Simultaneous Determination of Cholesterol and Cholesterol Oxides in Milk by RP-HPLC-DAD
Autor: Bauer, Luciana C. ; Santana, Débora de A. ; Macedo, Marianne dos S. ; Torres, Alexandre G. ; Souza, Nilson E. de ; Simionato, Julliana I.
Assuntos: CHEMISTRY, MULTIDISCIPLINARY
É parte de: Journal of the Brazilian Chemical Society, 2014-01, Vol.25 (1), p.161-168
Descrição: The aim of the present work was to develop and validate a reversed-phase highperformance liquid chromatography with diode array detection (RP-HPLC-DAD) method for determination of cholesterol and cholesterol oxides extracted from milk samples. The method consisted of direct samples saponification and analytes extraction, followed by their determination using RP-HPLC-DAD. Suitable analytical selectivity, linearity and range of application, intra-assay and intermediary precision, recovery, detection and quantification limits (LOD and LOQ) were achieved and, the results showed that the developed RP-HPLC-DAD method can be applied to routine determination of cholesterol and cholesterol oxides in milk samples.
Editor: Sociedade Brasileira de Química
Idioma: Inglês;Português

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