Idioma:

Forkhead box protein 3 in human nasal polyp regulatory T cells is regulated by the protein suppressor of cytokine signaling 3

Lan, Feng, MD ; Zhang, Nan, MD, PhD ; Zhang, Jie, MBBS ; Krysko, Olga, MD, PhD ; Zhang, Quanbo, MD, PhD ; Xian, Junming, MD, PhD ; Derycke, Lara, PhD ; Qi, Yanyu, MD ; Li, Kai, MD ; Liu, Shixi, MD, PhD ; Lin, Ping, PhD ; Bachert, Claus, MD, PhD

Journal of allergy and clinical immunology, 2013-12, Vol.132 (6), p.1314-1321.e3 [Periódico revisado por pares]

New York, NY: Mosby, Inc

Texto completo disponível

Citações Citado por

Enviar para

Título:
Forkhead box protein 3 in human nasal polyp regulatory T cells is regulated by the protein suppressor of cytokine signaling 3
Autor: Lan, Feng, MD ; Zhang, Nan, MD, PhD ; Zhang, Jie, MBBS ; Krysko, Olga, MD, PhD ; Zhang, Quanbo, MD, PhD ; Xian, Junming, MD, PhD ; Derycke, Lara, PhD ; Qi, Yanyu, MD ; Li, Kai, MD ; Liu, Shixi, MD, PhD ; Lin, Ping, PhD ; Bachert, Claus, MD, PhD
Assuntos: Adolescent ; Adult ; airway disease ; Allergy and Immunology ; Antibiotics ; Antigens ; Asthma ; Biological and medical sciences ; Carrier Proteins ; Cell Line, Tumor ; Chronic Disease ; Cytokines ; Female ; Forkhead box protein 3 ; Forkhead Transcription Factors - genetics ; Forkhead Transcription Factors - metabolism ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Gene Expression Regulation - genetics ; Gene Expression Regulation - immunology ; Growth hormones ; Humans ; Kinases ; Klinisk medicin ; Male ; Medical sciences ; Medicin och hälsovetenskap ; Middle Aged ; Nasal Mucosa - immunology ; nasal polyps ; Nasal Polyps - immunology ; Oto-rino-laryngologi ; Phosphorylation ; Proteins ; Rhinitis - immunology ; RNA, Small Interfering - genetics ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis ; signal transducer and activator of transcription 3 ; Sinusitis - immunology ; STAT3 Transcription Factor - metabolism ; suppressor of cytokine signaling 3 ; Suppressor of Cytokine Signaling 3 Protein ; Suppressor of Cytokine Signaling Proteins - genetics ; Suppressor of Cytokine Signaling Proteins - metabolism ; T-Lymphocytes, Regulatory - immunology ; Technological change ; Transgenes - genetics ; Young Adult
É parte de: Journal of allergy and clinical immunology, 2013-12, Vol.132 (6), p.1314-1321.e3
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
Descrição: Background In patients with persistent upper airway inflammation, the number of forkhead box protein 3 (Foxp3)+ regulatory T (Treg) cells is reduced, but the regulation of Foxp3 expression in Treg cells is poorly understood. Objective We investigated the interaction between suppressor of cytokine signaling 3 (SOCS3) and Foxp3 expression in the airway mucosa. Methods Expression of SOCS3 and Foxp3 was measured in tissue from patients with chronic rhinosinusitis with nasal polyps (CRSwNP) and control tissue. Coexpression of SOCS3 and Foxp3 was evaluated in PBMCs and in tissue from patients with CRSwNP. We also switched off and overexpressed SOCS3 in tissue from patients with CRSwNP and in pancreatic carcinoma epithelial-like cell line (PANC-1) cells and examined the effect on Foxp3 expression. Results SOCS3 gene and protein expression was upregulated in inflammatory cells in airway mucosa, whereas Foxp3 gene and protein expression was downregulated. Mucosal Treg cells coexpressed both proteins. Switching off the expression of SOCS3 in human airway mucosa resulted in Foxp3 upregulation, whereas inducing it in PANC-1 cells led to Foxp3 downregulation. We also found that phosphorylation of signal transducer and activator of transcription (STAT) 3 was decreased in inflamed mucosa, and we hypothesized that SOCS3 was responsible. Phosphorylation of STAT3 increased on silencing SOCS3 expression in inflamed mucosa and decreased on SOCS3 plasmid transfection in PANC-1 cells. Conclusion For the first time, we demonstrate that SOCS3 and Foxp3 are coexpressed in Treg cells in human nasal mucosa and that SOCS3 negatively regulates Foxp3 expression in human airway mucosa, possibly through phosphorylation of STAT3. Hence SOCS3 could be a potential target for restoring Foxp3 expression in Treg cells in patients with persistent mucosal inflammation.
Editor: New York, NY: Mosby, Inc
Idioma: Inglês

Links

Voltar para lista de resultados

Anterior Resultado 6 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Forkhead box protein 3 in human nasal polyp regulatory T cells is regulated by the protein suppressor of cytokine signaling 3

Lan, Feng, MD ; Zhang, Nan, MD, PhD ; Zhang, Jie, MBBS ; Krysko, Olga, MD, PhD ; Zhang, Quanbo, MD, PhD ; Xian, Junming, MD, PhD ; Derycke, Lara, PhD ; Qi, Yanyu, MD ; Li, Kai, MD ; Liu, Shixi, MD, PhD ; Lin, Ping, PhD ; Bachert, Claus, MD, PhD

New York, NY: Mosby, Inc

Buscando em bases de dados remotas. Favor aguardar.