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Sensitive HIV-1 DNA Pol Next-Generation Sequencing for the Characterisation of Archived Antiretroviral Drug Resistance

Botha, Johannes C ; Byott, Matthew ; Spyer, Moira J ; Grant, Paul R ; Gärtner, Kathleen ; Chen, Wilson X ; Burton, James ; Bamford, Alasdair ; Waters, Laura J ; Giaquinto, Carlo ; Turkova, Anna ; Vavro, Cindy L ; Nastouli, Eleni

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Título:
Sensitive HIV-1 DNA Pol Next-Generation Sequencing for the Characterisation of Archived Antiretroviral Drug Resistance
Autor: Botha, Johannes C ; Byott, Matthew ; Spyer, Moira J ; Grant, Paul R ; Gärtner, Kathleen ; Chen, Wilson X ; Burton, James ; Bamford, Alasdair ; Waters, Laura J ; Giaquinto, Carlo ; Turkova, Anna ; Vavro, Cindy L ; Nastouli, Eleni
Assuntos: drug resistance ; HIV-1 ; HIV-1 DNA ; NGS ; provirus ; sanger
Notas: Viruses , 15 (9) , Article 1811. (2023)
Descrição: Modern HIV-1 treatment effectively suppresses viral amplification in people living with HIV. However, the persistence of HIV-1 DNA as proviruses integrated into the human genome remains the main barrier to achieving a cure. Next-generation sequencing (NGS) offers increased sensitivity for characterising archived drug resistance mutations (DRMs) in HIV-1 DNA for improved treatment options. In this study, we present an ultra-sensitive targeted PCR assay coupled with NGS and a robust pipeline to characterise HIV-1 DNA DRMs from buffy coat samples. Our evaluation supports the use of this assay for Pan-HIV-1 analyses with reliable detection of DRMs across the HIV-1 Pol region. We propose this assay as a new valuable tool for monitoring archived HIV-1 drug resistance in virologically suppressed individuals, especially in clinical trials investigating novel therapeutic approaches.
Editor: MDPI AG
Data de criação/publicação: 2023
Idioma: Inglês

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