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Diversity of bacteria and archaea associated with the degradation of recalcitrant compounds

G S Bellincanta A L Conationi; C R Nakayama; F F Piza; D F Rodrigues; F Talarico; V Oliveira; Rosana Filomena Vazoller; G P Manfio; Vivian Helena Pellizari; Congresso do Instituto de Ciências Biomédicas (3. 2001 São Paulo)

Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2001

São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP 2001

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  • Título:
    Diversity of bacteria and archaea associated with the degradation of recalcitrant compounds
  • Autor: G S Bellincanta
  • A L Conationi; C R Nakayama; F F Piza; D F Rodrigues; F Talarico; V Oliveira; Rosana Filomena Vazoller; G P Manfio; Vivian Helena Pellizari; Congresso do Instituto de Ciências Biomédicas (3. 2001 São Paulo)
  • Assuntos: MICROBIOLOGIA
  • É parte de: Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2001
  • Descrição: Abstract: The current project will investigate the occurrence and diversity of Bacteria and Archaea Domains able to degrade OCLs and hydrocarbons in environmental samples, taken from Santos estuary. It will also assess the presence of methanogenic Archaea and anaerobic dehalogenating bacteria in biofilms, developed inside anaerobic bioreactors used to remove chlorinated compounds from wastewater. In this project, traditional culture-based and microscopic techniques, chemical analyses, and molecular methods will be combined to better link microbial structure and function. Classical methods to characterize aerobic and anaerobic cells (fermentative products, microscopic exams, presence of anaerobic cofactors and enzymes) will be associated with molecular techniques, such as the use of fluorescent probes for hybridization with nucleic acids present in whole cells, in order to visualize single cells constituting specific populations in samples and the spatial position of various members in microbial communities. In addition, methanogenic cells and sulphate reducing bacteria will be detected by 16S rRNA probes and PCR amplification of 16S rDNA and DGGE. Molecular biology techniques will also be to determine the presence of specific catabolic genotype in the studied area. This work was supported by the State of São Paulo Research Foundation
  • Editor: São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP
  • Data de criação/publicação: 2001
  • Formato: 1 v. pôster 187.
  • Idioma: Inglês

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