skip to main content
Idioma:
Tipo de recurso Mostra resultados com: Mostra resultados com: Índice

Effects of prenatal exposure to ethanol on development of substance P and tyrosine hydroxylase immunoreactive containing neurons

A G Commodaro Gerson Chadi; Reunião Anual da Federação de Sociedades de Biologia Experimental - FeSBE (16. 2001 Caxumbu)

Resumos Caxambu: Federação de Sociedades de Biologia Experimental, 2001

Caxambu Federação de Sociedades de Biologia Experimental 2001

Item não circula. Consulte sua biblioteca.(Acessar)

  • Título:
    Effects of prenatal exposure to ethanol on development of substance P and tyrosine hydroxylase immunoreactive containing neurons
  • Autor: A G Commodaro
  • Gerson Chadi; Reunião Anual da Federação de Sociedades de Biologia Experimental - FeSBE (16. 2001 Caxumbu)
  • Assuntos: ANATOMIA
  • É parte de: Resumos Caxambu: Federação de Sociedades de Biologia Experimental, 2001
  • Descrição: Objetivo: Objective: Fetal alcohol exposure can disturb neuronal development. However the lacking of adequate methods for quantification does not allow a detailed analysis of the impairment. The present work analysed the effects of chronic alcohol consumption during pregnancy on the development of substance P (SP) and tyrosine hydroxylase (TH) central nervous system neurons using the advantage of quantitative image analysis in combination to imunohistochemistry. Métodos e Resultados: Method and Results: Female Wistar rats were divided into two weight-matched groups. One group was given a high protein liquid diet ad libitum containing 37.5% ethanol-derived calories (6.7% v/v) during 21 days. Another group received the same volume of liquid diet containing isocaloric amount of the maltose-dextrin substituted for ethanol. On gestation day 19, embryos were removed and their brains processed for substance P and tyrosine hydroxylase immunohistochemistry. Every five section was systematically sampled and analysed using a Kontron/Zeiss KS-400 Image Analyser. The SP immunoreactive area of the subplate cortical of the cingulate cortex decreased [616.34±51.75 (pair-fed offspring), 446.80±44.5 (EtOH-exposed offspring), p<0.05, n=7 (unpaired t-Test)] The hippocampus of the embryos of the diet alcohol group showed significant lower SP immunoreactive area [887.99±31.75(pair-fed offspring), 607.28±11.87(EtOH-exposed offspring), p<0.001, n=7 (unpaired t-Test)]. The TH
    immunoreactive area of the striatum decreased [4080.62±283.2 (pair-fed offspring), 2053.77±202.4 (EtOH-exposed offspring), p<0.001, n=7 (unpaired t-Test)] and pars compacta of the substantia nigra also decreased [8348.65±178.06 (pair-fed offspring), 6395.29±297.13 (EtOH-exposed offspring), p<0.001, n=7 (unpaired t-Test)] Conclusões: Conclusion: The data indicated that quantitative image analysis method combined to immunohistochemistry are powerful tools to study the neurotoxicity in the central nervous system. Chronic alcohol consumption during pregnancy impairs the development of specific neurotransmitter systems
  • Editor: Caxambu Federação de Sociedades de Biologia Experimental
  • Data de criação/publicação: 2001
  • Formato: 1 v. (várias paginações).
  • Idioma: Inglês
Links
Voltar para lista de resultados
Ir para página anteriorAnterior Resultado  7 AvançarIr para próxima página

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.