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Manipulating the mouse embryo a laboratory manual
Brigid Hogan
Plainview, N.Y. Cold Spring Harbor Laboratory Press 1994
Localização:
FMRP - Fac. Medicina de Ribeirão Preto
(616-092.9 H714m2 )
(Acessar)
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Título:
Manipulating the mouse embryo a laboratory manual
Autor:
Brigid Hogan
Assuntos:
Mice -- Genetic engineering -- Laboratory manuals
;
Transgenic mice -- Laboratory manuals
;
Mice as laboratory animals
;
ANIMAIS DE LABORATÓRIO
;
Mice -- embryology -- laboratory manuals
;
Genetic Engineering -- laboratory manuals
;
Mice, Transgenic -- genetics -- laboratory manuals
Notas:
Includes bibliographical references (p. 439-470) and index
Descrição:
Developmental genetics and embryology of the mouse: past, present and future -- Summary of mouse development -- Setting up a colony for the production of transgenic and mutant mice -- Recovery, culture and transfer of embryos a nd germ cells -- Invitro manipulation of preimplantation embryos -- Production of transgenic mice -- Isolation, culture and manipulation of embryonic stem cells -- Analysis of transgenic mice -- Techniques for visualizing genes , gene products and specialized celltypes
In vitro culture of eggs, embryos, primordial germ cells and teratocarcinoma cells -- Buffers and solutions -- Acidic tyrode solution for removing zonae -- Alkaline phosphatase buffers -- Alkaline phosphatase staining solution -- Avertin,anesthetic -- Bovine serum albumin (BSA) -- Cap++s/Mgp++s-free PBS -- Cap++s/Mgp++s-free tyrode ringer's saline (pH 7.6-7.7) -- Hyaluronidase -- NTM buffer (NTMT without tween 20) for in situ hybridization of intact embryos with RNAprobes -- NTMT buffer for in situ hybridization of intact embryos with RNA probes Pancreatin/trypsin solution for separating germ and tissue layers -- PBSMT for immunohistochemistry of whole mount embryos -- PBT -- Phenol/chloroform solution for isolating total RNA from mouse embryos or fetal tissues -- Phos phate-bufferedsaline (PBSö -- Pronase solution -- Saline/EDTA buffer plus glucose for isolation of germ cells and tissue culture -- 20X SSC -- 20X SSPE -- TE (tris/EDTA) buffer -- 0.25% trypsin in tris-saline for tissue culture -- Trypsin/EDTA solutions --Sources of information -- Genetic resources -- Books -- Journals Databases -- Software -- Suppliers -- Inbred, hybrid and random-bred mice -- Mouse genomic DNAs and RNAs -- Mouse genomic and cDNA libraries and clones -- Equipment and supplies
Editor:
Plainview, N.Y. Cold Spring Harbor Laboratory Press
Data de criação/publicação:
1994
Formato:
xvii, 497 p. ill. (some col.) 29 cm.
Idioma:
Inglês
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