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Molecular biology and biotechnology a guide for students

Helen Kreuzer Adrianne Massey; Helen Kreuzer

Washington, D.C ASM Press c2008

Disponible en EEL - Biotecnologia e Química    (577.2 K889ms 3ed. ) y otras localizaciones(Obténgalo)

  • Título:
    Molecular biology and biotechnology a guide for students
  • Autor: Helen Kreuzer
  • Adrianne Massey; Helen Kreuzer
  • Materias: Molecular Biology; Biotechnology -- methods; DNA -- genetics; Genetic Engineering -- methods; Biotechnology; Molecular biology; BIOLOGIA MOLECULAR; BIOTECNOLOGIA
  • Notas: Includes bibliographical references and index
  • Descripción: pt. I. Laying the foundation. An overview of biotechnology -- Cell properties and processes -- Genes, genetics, and geneticists -- An overview of molecular biology -- Recombinant DNA technology -- pt. II. classroom activities. A. DNA structure and function. Student Activity: constructing a paper DNA model -- Student activity: DNA replication -- Student activity: from genes to proteins -- Student activity: making antisense: regulating gene expression with RNA -- Student activity: Sizes of the Escherichia coli and human genomes -- Student activity: Extraction of bacterial DNA -- B. Manipulation and analysis of DNA. Student activity: DNA scissors -- Student activity: DNA goes to the races -- Student activity: restriction analysis of lambda DNA -- Student activity: recombinant paper plasmids -- Student activity: restriction analysis challenge -- Student activity: Detection ofspecific DNA sequences: pt. I. Fishing for DNA. Student activity: detection of specific DNA sequences: pt. II. Combining restriction and hybridization analysis. Student activity: detection of specific DNA sequences: pt. III. Southern hybridization -- Student activity: paper PCR -- Student activity: DNA sequencing: the terminators. Reading: chain terminators as antiviral drugs.
    C. Transfer of genetic information. Student activity: transformation of Escherichia coli -- Student activity: conjugative transfer of antibiotic resistance in Escherichia coli. Reading: gene transfer and the spread of antibiotic resistance -- Student activity: transduction of an antibiotic resistance gene -- Student activity: Agrobacterium tumefaciens: nature's plant genetic engineer -- D. Molecular biology and genetics. Student activity: an adventure in dog hair, pt. I -- Student activity: an adventure in dog hair, pt. II: Yellow labs -- Student activity: human molecular genetics. Reading: Molecular genetics of cancer -- Student activity: genetics in action -- E. Genomics. Student activity: comparing genomes excercise 1: STRs can cause an RFLP. Exercise 2: PCR can reveal differences at microsatellite loci. Reading: mitochondrial DNA -- Student activity: forensic DNA typing. Exercise 1. A mix-up at the hospital. Exercise 2. A paternity case. Excercise 3. The case of the bloody knife. Reading: DNA forensics: an archaeological case -- Student activity: mapping a disease gene. Reading: the human genome project: its science, applications, and issues -- Student activity: microarray analysis of genome expression. Reading: personal genomics.
    F. Bioinformatics and evolutionary analysis of proteins. Student activity: testing for amylase activity -- Student activity: electrophoresis of amylase samples -- Student activity: constructing an amylase evolutionary tree -- Student activity: exploring bioinformatics -- pt. III. Societal issues. Student activity: facing the forces: science, technology, and society. Reading: the nature of science -- Student activity: analyzing the issues of biotechnology: cloning. reading: are bioengineered foods safe? -- Studnet activity: the risks of technology: perception, reality, or both? Reading: biotechnology regulation in the United States -- Student activity: making decision when there is no right answer -- Student activity: analyzing ethical dilemmas in genetic testing. Reading: science, law, and politics -- Student activity: analyzing ethical dilemmas in gene therapy -- pt. IV. Appendixes. A. Worksheets -- B. Laboratory biosafety -- C. Aseptic technique
  • Editor: Washington, D.C ASM Press
  • Fecha de creación: c2008
  • Formato: x, 487 p ill. (some col.) 28 cm.
  • Idioma: Inglés
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