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Reusing wastewater from processing to produce single-cell protein using : Optimizing of culture conditions

Omar Santiago Pillaca Pullo André Moreni Lopes; Waldir D. Estela Escalante

Biotechnology Progress v.39, n.5, p.1-12, art. e3393, 2023

Nova Iorque Aiche 2023

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  • Título:
    Reusing wastewater from processing to produce single-cell protein using : Optimizing of culture conditions
  • Autor: Omar Santiago Pillaca Pullo
  • André Moreni Lopes; Waldir D. Estela Escalante
  • Assuntos: RESÍDUOS AGRÍCOLAS; Candida Sorboxylosa; Agro-Industrial Wastes; Coffee Wastewater (Cww); Factorial Fractional Design; Ph Variation; Single-Cell Protein (Scp)
  • É parte de: Biotechnology Progress v.39, n.5, p.1-12, art. e3393, 2023
  • Notas: Disponível em: https://doi.org/10.1002/btpr.3393. Acesso em: 23 nov. 2023
  • Descrição: Coffee is a crop of significant socioeconomic importance, and the reuse of agri-food by-products and biowaste has great potential across several industries. Coffee wastewater (CWW) is a valuable resource containing essential nutrients that can be utilized by Candida sorboxylosa for single-cell protein (SCP) production. This utilization contributes to mitigating the negative impacts of agro-industrial waste. The optimization of culture conditions using the design of experiments (DoE) technique is crucial in understanding the environmental factors influencing metabolite production. In our study, the DoE technique was employed to analyze culture conditions, including room temperature, pH 8.4, agitation at 200 rpm, a headspace of 60% (v/v), and an inoculum of 0.75 DO600nm over 28-h period. This approach resulted in a remarkable SCP yield of 64.4% and dry cell weight (DCW) of 2.26 g/L. It is noteworthy that there is no literature reporting SCP production under alkaline pH conditions in yeast. Interestingly, our work demonstrated that an alkaline pH of 8.4 significantly influenced SCP production by C. sorboxylosa. The DoE technique proved to be an efficient statistical tool for optimizing culture conditions, offering several advantages, such as: (i) conducting cultures at room temperature to minimize unnecessary energy consumption; (ii) reducing the incubation time from 46 to 28 h, thereby enhancing overall productivity; (iii) achieving 1.7-fold increase in SCP yield compared to previous basal production levels.
  • Editor: Nova Iorque Aiche
  • Data de criação/publicação: 2023
  • Formato: p. 1-12.
  • Idioma: Inglês

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