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Simultaneous determination of anti-diabetic drugs

Sher, Nawab; Fatima, Nasreen; Perveen, Shahnaz; Siddiqui, Farhan Ahmed

Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e17394

Universidade de São Paulo. Faculdade de Ciências Farmacêuticas 2019-12-09

Acesso online

  • Título:
    Simultaneous determination of anti-diabetic drugs
  • Autor: Sher, Nawab; Fatima, Nasreen; Perveen, Shahnaz; Siddiqui, Farhan Ahmed
  • Assuntos: Metformin/Pharmacokinetics; High Pressure Liquid Chromatography; Urine/Plasma Methods; Determination/Prevention; Diabetes Mellitus/Drug Therapy; Drug Liberation/Drug Effects
  • É parte de: Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e17394
  • Descrição: A novel reverse phase, isocratic HPLC method is described to separate five anti-diabetic drugs i.e., glimepiride, metformin, sitagliptin, rosiglitazone and pioglitazone. Nucleosil C18 analytical column was used as stationary phase, while mobile phase consisted of acetonitrile:phosphate buffer: methanol (40/40/20, v/v) pH 2.0. Effluent was monitored at a flow rate 1 mL/min and detected at wavelength of 240 nm. This research produced excellent chromatography over a wide concentration range of 25‑10000 ng/mL. Sepprated and well resolved quantifiable peaks were obtained and test results were linear in this range. Correlation coefficient of more than 0.9990 was witnessed as well as Low %RSD values i.e., maximum 2.0% documented excellent precision of the method. Good recoveries from pharmaecutical (99-101%), urine and plasma samples (>96%) in a range of concentrtion granted very good linearity, accuracy and precision. The projected method has satisfactory applications in quality control of these molecules as well as quantification of these molecules in urine and plasma samples.
  • Títulos relacionados: https://www.revistas.usp.br/bjps/article/view/180916/167965
  • Editor: Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
  • Data de criação/publicação: 2019-12-09
  • Formato: Adobe PDF
  • Idioma: Inglês

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