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Preferential histochemical staining of protoplasmic and fibrous astrocytes in rat CNS with GFAP antibodies using different fixatives

Shehab, Safa A.S. ; Cronly-Dillon, John R. ; Nona, Shmaiel N. ; Stafford, Carole A.

Brain research, 1990-06, Vol.518 (1-2), p.347-352 [Periódico revisado por pares]

Netherlands: Elsevier B.V

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Título:
Preferential histochemical staining of protoplasmic and fibrous astrocytes in rat CNS with GFAP antibodies using different fixatives
Autor: Shehab, Safa A.S. ; Cronly-Dillon, John R. ; Nona, Shmaiel N. ; Stafford, Carole A.
Assuntos: Animals ; Anti-glial fibrillary acidic protein ; Antibodies ; Antibodies, Monoclonal ; Astrocyte ; Astrocytes - cytology ; Brain - cytology ; Ethanol ; Fixation ; Formaldehyde ; Glial Fibrillary Acidic Protein - analysis ; Glial Fibrillary Acidic Protein - immunology ; Histological Techniques ; Immunohistochemistry ; Polymers ; Rat ; Rats ; Rats, Inbred Strains ; Spinal Cord - cytology
É parte de: Brain research, 1990-06, Vol.518 (1-2), p.347-352
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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Descrição: Serial sections of rat brain and spinal cord were fixed in either acid-alcohol or 4% paraformaldehyde, and stained for visualization of astrocytes using GFAP antibodies. With paraformaldehyde, GFAP-positive astrocytes were visualized almost exclusively in the grey matter of all above tissues. In sharp contrast, acid-alcohol treatment gave intensely stained GFAP-containing astrocytes in the white matter. Since fibrous astrocytes are mainly located in the white matter and protoplasmic astrocytes are located in the grey matter, it is concluded that acid-alcohol is a good fixative for fibrous astrocytes while paraformaldehyde is a better fixative for protoplasmic astrocytes.
Editor: Netherlands: Elsevier B.V
Idioma: Inglês

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Preferential histochemical staining of protoplasmic and fibrous astrocytes in rat CNS with GFAP antibodies using different fixatives

Shehab, Safa A.S. ; Cronly-Dillon, John R. ; Nona, Shmaiel N. ; Stafford, Carole A.

Netherlands: Elsevier B.V

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