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Size of the Ovulatory Follicle Dictates Spatial Differences in the Oviductal Transcriptome in Cattle

Gonella-Diaza, Angela María ; Andrade, Sónia Cristina da Silva ; Sponchiado, Mariana ; Pugliesi, Guilherme ; Mesquita, Fernando Silveira ; Van Hoeck, Veerle ; Strefezzi, Ricardo de Francisco ; Gasparin, Gustavo R ; Coutinho, Luiz L ; Binelli, Mario Kues, Wilfried A.

PloS one, 2015-12, Vol.10 (12), p.e0145321-e0145321 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Size of the Ovulatory Follicle Dictates Spatial Differences in the Oviductal Transcriptome in Cattle
Autor: Gonella-Diaza, Angela María ; Andrade, Sónia Cristina da Silva ; Sponchiado, Mariana ; Pugliesi, Guilherme ; Mesquita, Fernando Silveira ; Van Hoeck, Veerle ; Strefezzi, Ricardo de Francisco ; Gasparin, Gustavo R ; Coutinho, Luiz L ; Binelli, Mario
Kues, Wilfried A.
Assuntos: Abundance ; Animal lactation ; Animal sciences ; Animals ; Apoptosis ; Biomarkers - metabolism ; Bos taurus indicus ; Cattle ; Cell growth ; Corpus luteum ; Embryos ; Estrous Cycle - physiology ; Estrus cycle ; Extracellular matrix ; Female ; Gene expression ; Genes ; Genetic aspects ; Gonadotropin-releasing hormone ; Growth factors ; High-Throughput Nucleotide Sequencing - methods ; Immunoenzyme Techniques ; Immunohistochemistry ; Insulin ; Insulin-like growth factors ; Ovarian Follicle - cytology ; Ovarian Follicle - metabolism ; Ovaries ; Oviduct ; Oviducts - cytology ; Oviducts - metabolism ; Ovulation ; Oxidative stress ; Physiological aspects ; Pregnancy ; Proteins ; Real-Time Polymerase Chain Reaction ; Receptors ; Regional analysis ; Reverse Transcriptase Polymerase Chain Reaction ; Ribonucleic acid ; RNA ; RNA, Messenger - genetics ; Rodents ; Sex ; Signaling ; Spatial distribution ; Sperm ; Steroid hormones ; Steroids ; Transcription ; Transcriptome ; Zoology
É parte de: PloS one, 2015-12, Vol.10 (12), p.e0145321-e0145321
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the experiments: AMGD GP FSM MB. Performed the experiments: AMGD MS RFS GG LLC. Analyzed the data: AMGD SCSA LLC MB. Contributed reagents/materials/analysis tools: LLC RFS MB. Wrote the paper: AMGD GP VV MB.
Competing Interests: The authors have declared that no competing interests exist.
Descrição: In cattle, molecular control of oviduct receptivity to the embryo is poorly understood. Here, we used a bovine model for receptivity based on size of the pre-ovulatory follicle to compare oviductal global and candidate gene transcript abundance on day 4 of the estrous cycle. Growth of the pre-ovulatory follicle (POF) of Nelore (Bos indicus) cows was manipulated to produce two groups: large POF large corpus luteum (CL) group (LF-LCL; greater receptivity) and small POF-small CL group (SF-SCL). Oviductal samples were collected four days after GnRH-induced ovulation. Ampulla and isthmus transcriptome was obtained by RNA-seq, regional gene expression was assessed by qPCR, and PGR and ERa protein distribution was evaluated by immunohistochemistry. There was a greater abundance of PGR and ERa in the oviduct of LF-LCL animals thus indicating a greater availability of receptors and possibly sex steroids stimulated signaling in both regions. Transcriptomic profiles indicated a series of genes associated with functional characteristics of the oviduct that are regulated by the periovulatory sex steroid milieu and that potentially affect oviductal receptivity and early embryo development. They include tissue morphology changes (extra cellular matrix remodeling), cellular changes (proliferation), and secretion changes (growth factors, ions and metal transporters), and were enriched for the genes with increased expression in the LF-LCL group. In conclusion, differences in the periovulatory sex steroid milieu lead to different oviductal gene expression profiles that could modify the oviductal environment to affect embryo survival and development.
Editor: United States: Public Library of Science
Idioma: Inglês

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Size of the Ovulatory Follicle Dictates Spatial Differences in the Oviductal Transcriptome in Cattle

Gonella-Diaza, Angela María ; Andrade, Sónia Cristina da Silva ; Sponchiado, Mariana ; Pugliesi, Guilherme ; Mesquita, Fernando Silveira ; Van Hoeck, Veerle ; Strefezzi, Ricardo de Francisco ; Gasparin, Gustavo R ; Coutinho, Luiz L ; Binelli, Mario Kues, Wilfried A.

United States: Public Library of Science

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