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Perspectives on label-free microscopy of heterogeneous and dynamic biological systems

Pham, Dan L ; Gillette, Amani A ; Riendeau, Jeremiah ; Wiech, Kasia ; Guzman, Emmanuel Contreras ; Datta, Rupsa ; Skala, Melissa C

Journal of biomedical optics, 2025-12, Vol.29 (Suppl 2), p.S22702-S22702 [Periódico revisado por pares]

United States: Society of Photo-Optical Instrumentation Engineers

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  • Título:
    Perspectives on label-free microscopy of heterogeneous and dynamic biological systems
  • Autor: Pham, Dan L ; Gillette, Amani A ; Riendeau, Jeremiah ; Wiech, Kasia ; Guzman, Emmanuel Contreras ; Datta, Rupsa ; Skala, Melissa C
  • Assuntos: Animals ; Flow Cytometry ; Histological Techniques ; Image Processing, Computer-Assisted ; Microscopy ; Special Issue Honoring Gabriel Popescu, Pioneer in Biomedical Optics
  • É parte de: Journal of biomedical optics, 2025-12, Vol.29 (Suppl 2), p.S22702-S22702
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Advancements in label-free microscopy could provide real-time, non-invasive imaging with unique sources of contrast and automated standardized analysis to characterize heterogeneous and dynamic biological processes. These tools would overcome challenges with widely used methods that are destructive (e.g., histology, flow cytometry) or lack cellular resolution (e.g., plate-based assays, whole animal bioluminescence imaging). This perspective aims to (1) justify the need for label-free microscopy to track heterogeneous cellular functions over time and space within unperturbed systems and (2) recommend improvements regarding instrumentation, image analysis, and image interpretation to address these needs. Three key research areas (cancer research, autoimmune disease, and tissue and cell engineering) are considered to support the need for label-free microscopy to characterize heterogeneity and dynamics within biological systems. Based on the strengths (e.g., multiple sources of molecular contrast, non-invasive monitoring) and weaknesses (e.g., imaging depth, image interpretation) of several label-free microscopy modalities, improvements for future imaging systems are recommended. Improvements in instrumentation including strategies that increase resolution and imaging speed, standardization and centralization of image analysis tools, and robust data validation and interpretation will expand the applications of label-free microscopy to study heterogeneous and dynamic biological systems.
  • Editor: United States: Society of Photo-Optical Instrumentation Engineers
  • Idioma: Inglês
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