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Brain-targeted delivery of protein using chitosan- and RVG peptide-conjugated, pluronic-based nano-carrier

Kim, Ja-Young ; Choi, Won Il ; Kim, Young Ha ; Tae, Giyoong

Biomaterials, 2013-01, Vol.34 (4), p.1170-1178 [Periódico revisado por pares]

Netherlands: Elsevier Ltd

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Título:
Brain-targeted delivery of protein using chitosan- and RVG peptide-conjugated, pluronic-based nano-carrier
Autor: Kim, Ja-Young ; Choi, Won Il ; Kim, Young Ha ; Tae, Giyoong
Assuntos: Advanced Basic Science ; Animals ; BBB ; beta-Galactosidase - administration & dosage ; beta-Galactosidase - pharmacokinetics ; Brain ; Brain - metabolism ; Brain - pathology ; Chitosan ; Chitosan - chemistry ; Dentistry ; Diffusion ; Diseases ; Drug delivery systems ; Glycoproteins - pharmacokinetics ; Imaging ; Male ; Mice ; Mice, Inbred C3H ; Mice, Nude ; Nanocapsules - administration & dosage ; Nanocapsules - chemistry ; Nanostructure ; Peptides ; Peptides - pharmacokinetics ; Pluronic ; Poloxamer - pharmacokinetics ; Proteins ; Rabies virus ; Rabies virus - chemistry ; Rabies virus glycoprotein (RVG) ; Tissue Distribution ; β-galactosidase
É parte de: Biomaterials, 2013-01, Vol.34 (4), p.1170-1178
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Abstract Brain-targeted delivery of drug or imaging agent is hard to achieve efficiently due to the infiltrative nature of the blood-brain barrier (BBB). Moreover, delivery of therapeutic proteins to brain tissue is further limited by the size and physic-chemical properties of proteins. In this work, we developed a chitosan-conjugated Pluronic-based nano-carrier with a specific target peptide for the brain (rabies virus glycoprotein; RVG29) and applied for the protein delivery to the brain. The in-vivo brain accumulation of the nano-carrier in mice followed i.v injection was optically monitored with Cy5.5-conjugation to the nano-carrier, and the result showed that the Pluronic-based nano-carrier conjugated with both chitosan and the peptide was very efficient for the accumulation in brain tissue and was remarkably better than the nano-carrier conjugated with the peptide only. β-galactosidase, a model protein, was also delivered and accumulated efficiently in the brain by loading in the nano-carrier, analyzed by the bio-distribution of β-galactosidase. The delivered protein in the brain also maintained its bioactivity. Therefore, RVG29- and chitosan-conjugated Pluronic-based nano-carrier could be potentially useful for the diagnosis and therapy of brain diseases.
Editor: Netherlands: Elsevier Ltd
Idioma: Inglês

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Brain-targeted delivery of protein using chitosan- and RVG peptide-conjugated, pluronic-based nano-carrier

Kim, Ja-Young ; Choi, Won Il ; Kim, Young Ha ; Tae, Giyoong

Netherlands: Elsevier Ltd

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