Idioma:

Nano-fluorescent quantum dots as substrates for determination of ribavirin in pharmaceuticals and human plasma as well as monitoring of its kinetic interaction with salmon sperm DNA

Abdel Hakiem, Ahmed Faried ; Boushra, John M. ; Noureldeen, Deena A. M. ; Lashien, Adel S. ; Attia, Tamer Z.

Journal of Analytical Science and Technology, 2023-12, Vol.14 (1), p.26-16, Article 26 [Periódico revisado por pares]

Singapore: Springer Nature Singapore

Texto completo disponível

Citações Citado por

Enviar para

Título:
Nano-fluorescent quantum dots as substrates for determination of ribavirin in pharmaceuticals and human plasma as well as monitoring of its kinetic interaction with salmon sperm DNA
Autor: Abdel Hakiem, Ahmed Faried ; Boushra, John M. ; Noureldeen, Deena A. M. ; Lashien, Adel S. ; Attia, Tamer Z.
Assuntos: Analytical Chemistry ; Binding ; Binding interaction kinetics to ssDNA ; Biomolecules ; Blood plasma ; Carbon ; Carbon dots ; Characterization and Evaluation of Materials ; Chemical synthesis ; Chemistry ; Chemistry and Materials Science ; Commercial capsules and spiked human plasma ; Deoxyribonucleic acid ; Determination of ribavirin ; DNA ; Flourescence spectroscopy ; Fluorescence ; Fluorescence spectroscopy ; Microorganisms ; Monitoring ; Monitoring/Environmental Analysis ; Phosphorus ; Phosphorus and nitrogen co-doped carbon quantum dots ; Quantum dots ; Quenching ; Rate constants ; Research Article ; Ribavirin ; Salmon ; Saxagliptin ; Spectrum analysis ; Sperm ; Spermatozoa ; Substrates ; Turning off (quenching) and turning on (peeling off)
É parte de: Journal of Analytical Science and Technology, 2023-12, Vol.14 (1), p.26-16, Article 26
Descrição: Ribavirin (RIB) was successfully determined by fluorescence spectroscopy upon its quenching to environment friendly phosphorus and nitrogen co-doped carbon quantum dots (PNQDs). Different analytical parameters affecting the fluorescence spectra have been optimized and validated in accordance to the ICH guidelines. The proposed method has provided an efficient tracing of the interaction between RIB molecules and the synthesized QDs in an acidic medium (off-mode). The RIB molecules have shown excellent sensitivity by quenching of the emission band at 401 nm upon excitation at 245 nm throughout a linear range of 0.06–10.00 µg/mL with detection and quantitation limits down to 14.00 and 40.00 ng/mL, respectively. The quenching mode was proven to be static in raw samples and samples extracted of spiked plasma for quenching rate constants of 1.30 × 10 12 L M −1 S −1 and 1.73 × 10 12 L M −1 S −1 , respectively. The proposed method has been successfully applied for determination of RIB in the commercial capsules and spiked human plasma samples with good recovery percentages in between 102.00 and 103.00%. Interestingly, these carbon dots have been utilized as nano-fluorescent platforms for assessment of the binding interaction kinetics between the RIB molecules and salmon sperm DNA (ssDNA). This has been implemented through peeling off the RIB molecules from surface of the PNQDs upon successive addition of the ssDNA and hence fluorescence restoration (turning on). Consequently, this provides a successful monitoring of its antimicrobial potency. It was evidenced a strong binding interaction with a binding constant of 2.38 × 10 4 mol −1 /L. Significantly, this could open doors for an extended application for on-site monitoring of RIB as well as its interactions with biomolecules and microorganisms.
Editor: Singapore: Springer Nature Singapore
Idioma: Inglês

Voltar para lista de resultados

Anterior Resultado 6 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Nano-fluorescent quantum dots as substrates for determination of ribavirin in pharmaceuticals and human plasma as well as monitoring of its kinetic interaction with salmon sperm DNA

Abdel Hakiem, Ahmed Faried ; Boushra, John M. ; Noureldeen, Deena A. M. ; Lashien, Adel S. ; Attia, Tamer Z.

Singapore: Springer Nature Singapore

Buscando em bases de dados remotas. Favor aguardar.