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Photobiomodulation enhances the Th1 immune response of human monocytes

de Castro, Mayara Santos ; Miyazawa, Marta ; Nogueira, Ester Siqueira Caixeta ; Chavasco, Jorge Kleber ; Brancaglion, Gustavo Andrade ; Cerdeira, Cláudio Daniel ; Nogueira, Denismar Alves ; Ionta, Marisa ; Hanemann, João Adolfo Costa ; Brigagão, Maísa Ribeiro Pereira Lima ; Sperandio, Felipe Fornias

Lasers in medical science, 2022-02, Vol.37 (1), p.135-148 [Periódico revisado por pares]

London: Springer London

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Título:
Photobiomodulation enhances the Th1 immune response of human monocytes
Autor: de Castro, Mayara Santos ; Miyazawa, Marta ; Nogueira, Ester Siqueira Caixeta ; Chavasco, Jorge Kleber ; Brancaglion, Gustavo Andrade ; Cerdeira, Cláudio Daniel ; Nogueira, Denismar Alves ; Ionta, Marisa ; Hanemann, João Adolfo Costa ; Brigagão, Maísa Ribeiro Pereira Lima ; Sperandio, Felipe Fornias
Assuntos: Antiinfectives and antibacterials ; CD163 antigen ; CD80 antigen ; Cell Survival ; Cell viability ; Chemiluminescence ; Dentistry ; Fungicides ; Gene expression ; Humans ; Immune response ; Immunity ; Inflammation ; Interleukin 10 ; Interleukin 6 ; Lasers ; Lasers, Semiconductor ; Light therapy ; Lymphocytes T ; Macrophages ; Medicine ; Medicine & Public Health ; Microbicides ; Monocytes ; Nitric oxide ; Optical Devices ; Optics ; Original Article ; Parameters ; Photonics ; Quantum Optics ; Reactive oxygen species ; Reagents ; Reverse transcription ; Semiconductor lasers ; Tumor necrosis factor-α ; Variance analysis
É parte de: Lasers in medical science, 2022-02, Vol.37 (1), p.135-148
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: This study aims to evaluate the effects of photobiomodulation (PBM) on human monocytes, assessing the oxidative burst and ultimate fungicidal potential of these cells, as well as the gene expression at the mRNA level of CD68, CD80, CD163, CD204, IL-6, TNF-α and IL-10 in derived macrophages. Primary cultures of human monocytes were irradiated with an InGaAlP (660 nm)/GaAlAs (780 nm) diode laser (parameters: 40 mW, 0.04 cm 2 , 1 W/cm 2 ; doses: 200, 400 and 600 J/cm 2 ). Cells were submitted to the chemiluminescence assay, and a microbicidal activity assay against Candida albicans was performed. Reactive oxygen species (ROS) and nitric oxide (NO) production were measured, and cell viability was assessed by the exclusion method using 0.2% Trypan blue reagent. Irradiated monocytes were cultured for 72 h towards differentiation into macrophages. Total RNA was extracted, submitted to reverse transcription and real-time PCR. The results were analysed by ANOVA and the Tukey test ( α = 0.05). Irradiated monocytes revealed a significant increase in their intracellular and extracellular ROS ( P < 0.001). The 660 nm wavelength and 400 J/cm 2 dose were the most relevant parameters ( P < 0.001). The fungicidal capacity of the monocytes was shown to be greatly increased after PBM ( P < 0.001). PBM increased the expression of TNF-α ( P = 0.0302) and the production of NO ( P < 0.05) and did not impair monocyte viability. PBM induces a pro-inflammatory Th1-driven response in monocytes and macrophages.
Editor: London: Springer London
Idioma: Inglês

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Photobiomodulation enhances the Th1 immune response of human monocytes

London: Springer London

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