Idioma:

Macromolecular crowding links ribosomal protein gene dosage to growth rate in Vibrio cholerae

Soler-Bistué, Alfonso ; Aguilar-Pierlé, Sebastián ; Garcia-Garcerá, Marc ; Val, Marie-Eve ; Sismeiro, Odile ; Varet, Hugo ; Sieira, Rodrigo ; Krin, Evelyne ; Skovgaard, Ole ; Comerci, Diego J ; Rocha, Eduardo P C ; Mazel, Didier

BMC biology, 2020-04, Vol.18 (1), p.43-43, Article 43 [Revista revisada por pares]

England: BioMed Central Ltd

Texto completo disponible

Citas Citado por

Acciones

Título:
Macromolecular crowding links ribosomal protein gene dosage to growth rate in Vibrio cholerae
Autor: Soler-Bistué, Alfonso ; Aguilar-Pierlé, Sebastián ; Garcia-Garcerá, Marc ; Val, Marie-Eve ; Sismeiro, Odile ; Varet, Hugo ; Sieira, Rodrigo ; Krin, Evelyne ; Skovgaard, Ole ; Comerci, Diego J ; Rocha, Eduardo P C ; Mazel, Didier
Materias: Analysis ; Antibiotics ; Bacteria ; Bacterial chromosome ; Bacterial physiology ; Bacterial Proteins - metabolism ; Bacteriology ; Biochemistry, Molecular Biology ; Cell cycle ; Cholera toxin ; Chromosomes ; Cytoplasm ; Deoxyribonucleic acid ; DNA ; DNA biosynthesis ; DNA Replication ; DNA, Bacterial - physiology ; Dosage ; Fluidity ; Gene Dosage ; Gene expression ; Genes ; Genes, Bacterial ; Genetic aspects ; Genomes ; Genomics ; Growth ; Growth rate ; Homeostasis ; Life Sciences ; Macromolecular crowding ; Macromolecules ; Microbiology and Parasitology ; Molecular biology ; Optimization ; Physiological aspects ; Physiology ; Production capacity ; Protein biosynthesis ; Protein synthesis ; Proteins ; Relocation ; Replication ; Replication Origin ; Replication origins ; Ribosomal proteins ; Ribosomal Proteins - metabolism ; RNA polymerase ; Scientific equipment industry ; Transcription ; Transcription (Genetics) ; Translation (Genetics) ; Vibrio cholerae ; Vibrio cholerae - genetics ; Vibrio cholerae - growth & development ; Waterborne diseases
Es parte de: BMC biology, 2020-04, Vol.18 (1), p.43-43, Article 43
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descripción: In fast-growing bacteria, the genomic location of ribosomal protein (RP) genes is biased towards the replication origin (oriC). This trait allows optimizing their expression during exponential phase since oriC neighboring regions are in higher dose due to multifork replication. Relocation of s10-spc-α locus (S10), which codes for most of the RP, to ectopic genomic positions shows that its relative distance to the oriC correlates to a reduction on its dosage, its expression, and bacterial growth rate. However, a mechanism linking S10 dosage to cell physiology has still not been determined. We hypothesized that S10 dosage perturbations impact protein synthesis capacity. Strikingly, we observed that in Vibrio cholerae, protein production capacity was independent of S10 position. Deep sequencing revealed that S10 relocation altered chromosomal replication dynamics and genome-wide transcription. Such changes increased as a function of oriC-S10 distance. Since RP constitutes a large proportion of cell mass, lower S10 dosage could lead to changes in macromolecular crowding, impacting cell physiology. Accordingly, cytoplasm fluidity was higher in mutants where S10 is most distant from oriC. In hyperosmotic conditions, when crowding differences are minimized, the growth rate and replication dynamics were highly alleviated in these strains. The genomic location of RP genes ensures its optimal dosage. However, besides of its essential function in translation, their genomic position sustains an optimal macromolecular crowding essential for maximizing growth. Hence, this could be another mechanism coordinating DNA replication to bacterial growth.
Editor: England: BioMed Central Ltd
Idioma: Inglés

Enlaces

Volver a la lista de resultados

Anterior Resultado 2 Siguiente Ir a la siguiente página

Realización: Logos de Redes Sociales:

Macromolecular crowding links ribosomal protein gene dosage to growth rate in Vibrio cholerae

Soler-Bistué, Alfonso ; Aguilar-Pierlé, Sebastián ; Garcia-Garcerá, Marc ; Val, Marie-Eve ; Sismeiro, Odile ; Varet, Hugo ; Sieira, Rodrigo ; Krin, Evelyne ; Skovgaard, Ole ; Comerci, Diego J ; Rocha, Eduardo P C ; Mazel, Didier

England: BioMed Central Ltd

Buscando en bases de datos remotas, por favor espere