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Salivary antibody detection in epidemiological surveys: a pilot study after a mass vaccination campaign against rubella in São Paulo, Brazil

de Azevedo Neto, Raymundo Scares ; Richards, Alison ; Nokes, David James ; Silveira, Ana Silvia Barreiros ; Cohen, Bernard John ; Passos, Saulo Duarte ; de Souza, Vanda Akico Ueda Fick ; Brown, David W.G. ; Pannuti, Claudio Sergio ; Massad, Eduardo

Transactions of the Royal Society of Tropical Medicine and Hygiene, 1995, Vol.89 (1), p.115-118 [Peer Reviewed Journal]

Oxford: Elsevier Ltd

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Title:
Salivary antibody detection in epidemiological surveys: a pilot study after a mass vaccination campaign against rubella in São Paulo, Brazil
Author: de Azevedo Neto, Raymundo Scares ; Richards, Alison ; Nokes, David James ; Silveira, Ana Silvia Barreiros ; Cohen, Bernard John ; Passos, Saulo Duarte ; de Souza, Vanda Akico Ueda Fick ; Brown, David W.G. ; Pannuti, Claudio Sergio ; Massad, Eduardo
Subjects: Adolescent ; Antibodies, Viral - analysis ; Biological and medical sciences ; Brazil ; Brazil - epidemiology ; Child ; Child, Preschool ; Enzyme-Linked Immunosorbent Assay ; Human viral diseases ; Humans ; Immunoglobulin G - analysis ; Infant ; Infectious diseases ; Medical sciences ; Pilot Projects ; Radioimmunoassay ; rubella ; Rubella - epidemiology ; Rubella - prevention & control ; Rubella Vaccine ; Rubella virus - immunology ; Saliva - immunology ; salivary antibody ; Sensitivity and Specificity ; Tropical medicine ; Vaccination ; Viral diseases ; Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye
Is Part Of: Transactions of the Royal Society of Tropical Medicine and Hygiene, 1995, Vol.89 (1), p.115-118
Notes: istex:3DFC4871913B609E1D18115932D3065FA65EAA90
Immunology
Address for correspondence: Dr R. S. de Azevedo Neto, Departamento de Patologia da Faculdade de Medicina da USP, Avenida Douter Arnaldo 455, São Paulo, SP, 01246-903, Brazil.
ark:/67375/HXZ-FW5573NN-T
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Description: The sensitivity and specificity of salivary rubella antibody detection was investigated using samples collected from 301 children after a mass vaccination campaign in the state of São Paulo, Brazil. Saliva samples were collected by 2 different methods: directly dribbling into a container or using a commercial collecting device. Corresponding finger-prick blood samples were collected on filter paper. Rubella specific immunoglobulin G (IgG) was measured in saliva by antibody capture radioimmunoassay and in blood samples by indirect enzyme-linked immunosorbent assay. The detection of salivary rubella specific IgG showed good correlation with the detection of rubella antibody in the blood samples. For both collecting techniques the predictive value for a positive saliva test was >99% compared with the results from the blood tests. However, the predictive value for a negative saliva test was only 58.3% for a dribbled sample, compared to 100% for saliva collected using the commercial device. Moreover, collecting saliva by dribbling from children less than 4 years old was difficult. The detection of rubella specific IgG in saliva collected using a commercial device proved to be sensitive and specific in this epidemiological study, encouraging its more widespread application as a means of surveillance after mass vaccination.
Publisher: Oxford: Elsevier Ltd
Language: English

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Salivary antibody detection in epidemiological surveys: a pilot study after a mass vaccination campaign against rubella in São Paulo, Brazil

de Azevedo Neto, Raymundo Scares ; Richards, Alison ; Nokes, David James ; Silveira, Ana Silvia Barreiros ; Cohen, Bernard John ; Passos, Saulo Duarte ; de Souza, Vanda Akico Ueda Fick ; Brown, David W.G. ; Pannuti, Claudio Sergio ; Massad, Eduardo

Oxford: Elsevier Ltd

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