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Transfer of label from aspartate to malate by the cell-free extract of Sedum mexicanum leaves

Amagasa, Tadashi ; Yoshida, Seiichi

Plant and cell physiology, 1979-01, Vol.20 (7), p.1191-1197 [Periódico revisado por pares]

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Título:
Transfer of label from aspartate to malate by the cell-free extract of Sedum mexicanum leaves
Autor: Amagasa, Tadashi ; Yoshida, Seiichi
Assuntos: Aspartate ; Crassulacean acid metabolism (CAM) ; Malate ; Sedum mexicanum ; Transfer of label
É parte de: Plant and cell physiology, 1979-01, Vol.20 (7), p.1191-1197
Notas: F60
F
istex:3ED7C4FB066BE1BD436AD32A078C9C9E1F0F6A89
ark:/67375/HXZ-PMK4G9KT-K
ArticleID:20.7.1191
Descrição: The cell-free extract from leaves of Sedum mexicanum, a typical CAM plant, formed 14C-malate from 14C-aspartate in the presence of NAD. No reduction of NAD was observed during the reaction. Analysis of this reaction revealed that the transfer of label from l4C-aspartate to malate takes place by the action of malate dehydrogenase and aspartate aminotransferase, and the reaction was reversible in model experiments with commercial enzymes. Pitfalls in assessing data on dark 14CO2 fixation in CAM are discussed with reference to the transfer of label between malate and aspartate without actual synthesis.
Editor: Oxford University Press
Idioma: Inglês

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