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Evaluation of different methods for Plasmodia detection, in well defined population groups in an endemic area of Brazil

AVILA, S. L. M ; LEANDRO, M. C ; CARVALHO, N. B ; OLIVEIRA, M. S ; ARRUK, V. G ; SANCHEZ, M. C. A ; BOULOS, M ; FERREIRA, A. W

Revista do Instituto de Medicina Tropical de São Paulo, 1994-04, Vol.36 (2), p.157-162 [Peer Reviewed Journal]

São Paulo: Instituto de Medicina Tropical de São Paulo

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Title:
Evaluation of different methods for Plasmodia detection, in well defined population groups in an endemic area of Brazil
Author: AVILA, S. L. M ; LEANDRO, M. C ; CARVALHO, N. B ; OLIVEIRA, M. S ; ARRUK, V. G ; SANCHEZ, M. C. A ; BOULOS, M ; FERREIRA, A. W
Subjects: Adult ; Animals ; Antibodies, Protozoan - blood ; Biological and medical sciences ; Blood Cell Count - methods ; Brazil - epidemiology ; Female ; Fluorescent Antibody Technique ; Human protozoal diseases ; Humans ; Immunoglobulin A - blood ; Immunoglobulin G - blood ; Immunoglobulin M - blood ; Infectious diseases ; Malaria ; Malaria, Falciparum - blood ; Malaria, Falciparum - diagnosis ; Malaria, Falciparum - epidemiology ; Malaria, Vivax - blood ; Malaria, Vivax - diagnosis ; Malaria, Vivax - epidemiology ; Male ; Medical sciences ; Middle Aged ; Parasitic diseases ; Plasmodium - isolation & purification ; Protozoal diseases ; Sensitivity and Specificity ; TROPICAL MEDICINE
Is Part Of: Revista do Instituto de Medicina Tropical de São Paulo, 1994-04, Vol.36 (2), p.157-162
Notes: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Description: In Brazil, more than 500,000 new cases of malaria were notified in 1992. Plasmodium falciparum and P. vivax are the responsible species for 99.3% of the cases. For adequate treatment, precoce diagnosis is necessary. In this work, we present the results of the traditional Plasmodia detection method, thick blood film (TBF), and the results of alternative methods: Immunofluorescence assay (IFA) with polyclonal antibody and Quantitative Buffy Coat method (QBC) in a well defined population groups. The analysis were done in relation to the presence or absence of malaria clinical symptoms. Also different classes of immunoglobulins anti-P.falciparum were quantified for the global analysis of the results, mainly in the discrepant results. We concluded that alternative methods are more sensitive than TBF and that the association of epidemiological, clinical and laboratory findings is necessary to define the presence of malaria.
Publisher: São Paulo: Instituto de Medicina Tropical de São Paulo
Language: English;Portuguese

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Evaluation of different methods for Plasmodia detection, in well defined population groups in an endemic area of Brazil

AVILA, S. L. M ; LEANDRO, M. C ; CARVALHO, N. B ; OLIVEIRA, M. S ; ARRUK, V. G ; SANCHEZ, M. C. A ; BOULOS, M ; FERREIRA, A. W

São Paulo: Instituto de Medicina Tropical de São Paulo

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