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Rapid detection of Leishmania infantum infection in dogs: comparative study using an immunochromatographic dipstick rk39 test and direct agglutination

Mohebali, M ; Taran, M ; Zarei, Z

Veterinary parasitology, 2004-05, Vol.121 (3), p.239-245 [Periódico revisado por pares]

Netherlands: Elsevier B.V

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Título:
Rapid detection of Leishmania infantum infection in dogs: comparative study using an immunochromatographic dipstick rk39 test and direct agglutination
Autor: Mohebali, M ; Taran, M ; Zarei, Z
Assuntos: agglutination tests ; Agglutination Tests - veterinary ; Animals ; Antibodies, Protozoan - blood ; canine visceral leishmaniosis ; Cupressus ; diagnostic techniques ; Dipstick rk39 ; disease detection ; disease diagnosis ; disease reservoirs ; Dog ; dog diseases ; Dog Diseases - diagnosis ; Dog Diseases - parasitology ; Dogs ; Female ; immunochromatography ; immunologic techniques ; infection ; Iran ; Leishmania infantum ; Leishmania infantum - growth & development ; Leishmania infantum infection ; Leishmaniasis, Visceral - diagnosis ; Leishmaniasis, Visceral - parasitology ; Leishmaniasis, Visceral - veterinary ; Male ; Predictive Value of Tests ; rapid methods ; Reagent Kits, Diagnostic - parasitology ; Sensitivity and Specificity ; serodiagnosis ; visceral leishmaniasis ; zoonoses
É parte de: Veterinary parasitology, 2004-05, Vol.121 (3), p.239-245
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SourceType-Scholarly Journals-1
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Descrição: A rapid, sensitive and specific tool for detection of Leishmania infantum infection in dogs, would be highly desirable, because it would allow control interventions in endemic areas of Zoonotic visceral leishmaniosis (ZVL). In this study, we compared an immunochromatographic dipstick test with direct agglutination test (DAT) for detecting L. infantum infections in dogs from areas of ZVL endemic in Iran. The validity of the dipstick rk39 (Cypress Diagnostic Company, Belgium) for canine visceral leishmaniosis (CVL) was compared with a standard direct agglutination test on 116 clinically suspected dogs and 152 healthy controls from endemic areas of Ardabil and East Azerbaijan provinces, north-western of Iran for 1 year. A sensitivity of 70.9% and specificity of 84.9% were found at a 1:320 cut off titer when DAT confirmed cases were compared with healthy control. As the dipstick rk39 test is rapid, noninvasive and does not require much expertise or elaborate equipment, it can be used for screening and diagnosis of canine visceral leishmaniosis in remote endemic areas.
Editor: Netherlands: Elsevier B.V
Idioma: Inglês

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Rapid detection of Leishmania infantum infection in dogs: comparative study using an immunochromatographic dipstick rk39 test and direct agglutination

Mohebali, M ; Taran, M ; Zarei, Z

Netherlands: Elsevier B.V

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