Infectious Enveloped RNA Virus Antigenic Chimeras

• Autor: London, Steven D. ; Schmaljohn, Alan L. ; Dalrymple, Joel M. ; Rice, Charles M.
• Assuntos: Amino Acid Sequence ; Animals ; Antibodies, Monoclonal - immunology ; Antibodies, Viral - immunology ; Antigens, Surface - genetics ; Antigens, Surface - immunology ; Antigens, Viral - chemistry ; Antigens, Viral - genetics ; Base Sequence ; Biochemistry ; Biological and medical sciences ; Biotechnology ; Chimera ; Chimeras ; Cloning, Molecular ; Complementary DNA ; DNA Mutational Analysis ; Epitopes ; Fundamental and applied biological sciences. Psychology ; Glycoproteins ; Immunization ; Libraries ; Medical research ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - immunology ; Methods. Procedures. Technologies ; Mice ; Molecular Sequence Data ; Multidisciplinary Sciences ; Neutralization Tests ; Oligonucleotides ; Protein engineering ; Ribonucleic acid ; Rift Valley Fever - prevention & control ; Rift Valley fever virus ; Rift Valley fever virus - genetics ; Rift Valley fever virus - immunology ; RNA ; Science & Technology ; Science & Technology - Other Topics ; Sindbis Virus ; Sindbis Virus - genetics ; Sindbis Virus - immunology ; Virions ; Viruses
• É parte de: Proceedings of the National Academy of Sciences - PNAS, 1992-01, Vol.89 (1), p.207-211
• Notas: Medline
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• Descrição: Random insertion mutagenesis has been used to construct infectious Sindbis virus structural protein chimeras containing a neutralization epitope from a heterologous virus, Rift Valley fever virus. Insertion sites, permissive for recovery of chimeric viruses with growth properties similar to the parental virus, were found in the virion E2 glycoprotein and the secreted E3 glycoprotein. For the E2 chimeras, the epitope was expressed on the virion surface and stimulated a partially protective immune response to Rift Valley fever virus infection in vivo. Besides providing a possible approach for developing live attenuated vaccine viruses, insertion of peptide ligands into virion surface proteins may ultimately allow targeting of virus infection to specific cell types.
  
• Editor: WASHINGTON: National Academy of Sciences of the United States of America
  
• Idioma: Inglês