Antimicrobial Resistance Laboratory Network's multisite evaluation of the ThermoFisher Sensititre GN7F broth microdilution panel for antimicrobial susceptibility testing

• Autor: Bhatnagar, Amelia S ; Machado, María-José ; Patterson, Logan ; Anderson, Karen ; Abelman, Rebecca L ; Bateman, Allen ; Biggs, Angela ; Bumpus-White, Porscha ; Craft, Bradley ; Howard, Mondraya ; LaVoie, Stephen P ; Lonsway, David ; Sabour, Sarah ; Schneider, Annah ; Snippes-Vagnone, Paula ; Tran, Michael ; Torpey, David ; Valley, Ann ; Elkins, Christopher A ; Karlsson, Maria ; Brown, Allison C
• Simner, Patricia J.
• Assuntos: Acinetobacter baumannii ; Anti-Bacterial Agents - pharmacology ; Bacteriology ; Drug Resistance, Bacterial ; Humans ; Laboratories ; Microbial Sensitivity Tests ; Pseudomonas aeruginosa ; Pseudomonas Infections
• É parte de: Journal of clinical microbiology, 2023-12, Vol.61 (12), p.e0079923-e0079923
• Notas: ObjectType-Article-1
  SourceType-Scholarly Journals-1
  ObjectType-Feature-2
  content type line 23
  Present address: Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA
  Present address: Sentara Healthcare, Norfolk, Virginia, USA
  Present address: School of Medicine, University of North Carolina, Chapel Hill, North Carolina, USA
  Present address: BD, Sparks, Maryland, USA
  The authors declare no conflict of interest.
  
• Descrição: In 2017, the Centers for Disease Control and Prevention (CDC) established the Antimicrobial Resistance Laboratory Network to improve domestic detection of multidrug-resistant organisms. CDC and four laboratories evaluated a commercial broth microdilution panel. Antimicrobial susceptibility testing using the Sensititre GN7F (ThermoFisher Scientific, Lenexa, KS) was evaluated by testing 100 CDC and Food and Drug Administration AR Isolate Bank isolates [40 Enterobacterales (ENT), 30 (PSA), and 30 (ACB)]. We assessed multiple amounts of transfer volume (TV) between the inoculum and tubed 11-mL cation-adjusted Mueller-Hinton broth: 1 µL [tribe Proteeae (P-tribe) only] and 10, 30, and 50 µL, resulting in respective CFU per milliter of 1 × 10 , 1 × 10 , 3 × 10 , and 5 × 10 . Four TV combinations were analyzed: standard (STD) [1 µL (P-tribe) and 10 µL], enhanced standard (E-STD) [1 µL (P-tribe) and 30 µL], 30 µL, and 50 µL. Essential agreement (EA), categorical agreement, major error (ME), and very major error (VME) were analyzed by organism then TVs. For ENT, the average EA across laboratories was <90% for 7 of 15 β-lactams using STD and E-STD TVs. As TVs increased, EA increased (>90%), and VMEs decreased. For PSA, EA improved as TVs increased; however, MEs also increased. For ACB, increased TVs provided slight EA improvements; all TVs yielded multiple VMEs and MEs. For ENT and ACB, Minimum inhibitory concentrations (MICs) trended downward using a 1 or 10 µL TV; there were no obvious MIC trends by TV for PSA. The public health and clinical consequences of missing resistance warrant increased TV of 30 µL for the GN7F, particularly for P-tribe, despite being considered "off-label" use.
  
• Editor: United States: American Society for Microbiology
  
• Idioma: Inglês