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Hyperglycaemia enhances nitric oxide production in diabetes: a study from South Indian patients

Adela, Ramu ; Nethi, Susheel Kumar ; Bagul, Pankaj K ; Barui, Ayan K ; Mattapally, Saidulu ; Kuncha, Madhusudan ; Patra, Chitta R ; Reddy, P Naveen Chander ; Banerjee, Sanjay K Gomes, Aldrin V.

PloS one, 2015-04, Vol.10 (4), p.e0125270-e0125270 [Revista revisada por pares]

United States: Public Library of Science

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Hyperglycaemia enhances nitric oxide production in diabetes: a study from South Indian patients
Autor: Adela, Ramu ; Nethi, Susheel Kumar ; Bagul, Pankaj K ; Barui, Ayan K ; Mattapally, Saidulu ; Kuncha, Madhusudan ; Patra, Chitta R ; Reddy, P Naveen Chander ; Banerjee, Sanjay K
Gomes, Aldrin V.
Materias: Adult ; Aged ; Animals ; Atherosclerosis ; Biomedical materials ; Blood Glucose - metabolism ; Cardiovascular disease ; Chemistry ; Coronary artery ; Coronary Artery Disease - complications ; Coronary heart disease ; Coronary vessels ; Diabetes ; Diabetes mellitus ; Diabetes mellitus (non-insulin dependent) ; Diabetes Mellitus, Experimental - blood ; Diabetes Mellitus, Type 2 - blood ; Diabetes Mellitus, Type 2 - complications ; Diabetes Mellitus, Type 2 - metabolism ; Diabetes Mellitus, Type 2 - pathology ; Diabetics ; Endothelial cells ; Endothelium ; Fasting - blood ; Female ; Fructose ; Gene expression ; Glucose ; Glycated Hemoglobin A - metabolism ; Hemoglobin ; Human Umbilical Vein Endothelial Cells - metabolism ; Humans ; Hyperglycemia ; Hyperglycemia - complications ; Hypertension ; India ; Insulin ; Kinases ; Lipids ; Male ; Medical research ; Metabolism ; Middle Aged ; Nitric oxide ; Nitric Oxide - biosynthesis ; Nitric Oxide - blood ; Nitric Oxide Synthase Type II - genetics ; Nitric Oxide Synthase Type III - genetics ; Nitric-oxide synthase ; Patients ; Pharmaceutical sciences ; Pharmacology ; Rats ; Rats, Sprague-Dawley ; Rodents ; Transcriptional Activation ; Type 2 diabetes
Es parte de: PloS one, 2015-04, Vol.10 (4), p.e0125270-e0125270
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the experiments: RA SKB. Performed the experiments: RA SKN AKB CRP PKB MK SM. Analyzed the data: RA CRP SKB. Wrote the paper: RA SKB. Clinical characteristic measurement: PNCR RA.
Current Address: Drug Discovery Research Center, Translational Health Science and Technology Institute (THSTI), Faridabad, 121001, India
Competing Interests: The authors have declared that no competing interests exist.
Descripción: We have previously reported that increased glucose levels were associated with higher serum nitric oxide (NO) levels in fructose-fed insulin resistant rats. However, the relationship between hyperglycemia and serum NO level was not clear. Therefore, the present study was designed to find the association between hyperglycemia and serum NO levels in Type 2 diabetic (T2DM) patients and T2DM with cardiovascular complication. Endothelial cells (HUVEC) were treated with of D-glucose (10-100mM), and NO levels and NOS gene expression was measured. Hyperglycaemia was induced in Sprague-Dawley rats, and serum NO levels were measured after 8 weeks. For clinical evaluation, five groups of patients were recruited: Control (CT, n=48), Type 2 diabetes (T2DM, n=26), T2DM with hypertension (DMHT, n=46), Coronary artery diseases (CAD, n=29) and T2DM with coronary artery diseases (DMCD, n=38). NO (nitrite + nitrate) levels were measured from human serum. We found a significant (p<0.05) and dose-dependent increase in NO levels in HUVEC cells after 4 hours of high glucose exposure. eNOS and iNOS gene expression was increased in HUVEC cells after different concentrations and time periods of glucose treatment. We also observed significant (149.1 ± 25 μM, p<0.01) increase in serum NO levels in hyperglycaemic rats compared to control (76.6 ± 13.2 μM). Serum NO level was significantly higher in T2DM (111.8 μM (81.7-122.4), p<0.001) and DMCD patients ((129.4 μM (121.2-143.5), p <0.001) but not in CAD patients (76.4 μM (70.5-87)), as compared to control (68.2 μM (56.4-82.3)). We found significantly lower NO levels (83.5 μM (60.5-122.9)) in subjects suffering from diabetes since more than 5 years, compared to subjects (115.3 μM (75.2-127.1), p<0.001) with less than 5 years. In conclusion, high NO levels were observed in South Indian diabetic patients. Higher glucose levels in serum might be responsible for activation of endothelial cells to enhance NO levels.
Editor: United States: Public Library of Science
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Hyperglycaemia enhances nitric oxide production in diabetes: a study from South Indian patients

Adela, Ramu ; Nethi, Susheel Kumar ; Bagul, Pankaj K ; Barui, Ayan K ; Mattapally, Saidulu ; Kuncha, Madhusudan ; Patra, Chitta R ; Reddy, P Naveen Chander ; Banerjee, Sanjay K Gomes, Aldrin V.

United States: Public Library of Science

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